NFKBIZ Polyklonaler Antikörper

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• KD/KO Validated

NFKBIZ Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 14014-1-AP

Synonyme

IKBZ, IkB zeta, I-kappa-B-zeta, IkappaBzeta, IkappaB zeta

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 14014-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14014-1-AP (NFKBIZ antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 14014-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14014-1-AP (NFKBIZ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14014-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14014-1-AP (NFKBIZ Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14014-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14014-1-AP (NFKBIZ Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IF Staining of HeLa using 14014-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NFKBIZ antibody (14014-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, THP-1-Zellen, U-251-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

14014-1-AP bindet in WB, IHC, IF/ICC, ELISA NFKBIZ und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NFKBIZ fusion protein Ag5120
• Vollständiger Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta
• Berechnetes Molekulargewicht: 78 kDa
• Beobachtetes Molekulargewicht: 78 kDa, 64 kDa
• GenBank-Zugangsnummer: BC060800
• Gene symbol: NFKBIZ
• Gene ID (NCBI): 64332
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NFKBIZ, also named as NF-kappa-B inhibitor zeta, is a 718 amino acid protein, which contains 7 ANK repeats and is expressed at high levels in peripheral blood leukocytes and lung, at moderate levels in liver, placenta, and at low levels in spleen, kidney, skeletal muscle and heart. NFKBIZ exists as three isoforms and molecular weight of isoform three is a 64 kDa. The isoform one is 78 kDa protein and isoform two is a 68 kDa protein. NFKBIZ is involved in regulation of NF-kappa-B transcription factor complexes. It Inhibits NF-kappa-B activity without affecting its nuclear translocation upon stimulation.

Publikationen

Species	Application	Title
human	WB	Front Immunol; Single-Cell Transcriptome Analysis of Chronic Antibody-Mediated Rejection After Renal Transplantation.; Authors - Fanhua Kong
human	WB	Front Mol Neurosci; Silencing NUDT21 Attenuates the Mesenchymal Identity of Glioblastoma Cells via the NF-κB Pathway.; Authors - Jia-Cheng Lou
mouse	WB	Toxicol Appl Pharmacol; Tetrachloro-p-benzoquinone induces hepatic oxidative damage and inflammatory response, but not apoptosis in mouse: The prevention of curcumin.; Authors - Demei Xu
		Int J Biol Macromol; Charaterization and immunomodulatory activities of polysaccharide isolated from Pleurotus eryngii.; Authors - Duoduo Xu
rat	WB	J Surg Res; PMID: 25103639; Authors - Yu Zhang
mouse	IF	JCI Insight; The negative feedback loop of NF-κB/miR-376b/NFKBIZ in septic acute kidney injury.; Authors - Zhiwen Liu ; KD Validated