IkB Alpha Polyklonaler Antikörper

IkB Alpha Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

479

Rezensionen (2)

Kat-Nr. : 10268-1-AP

Synonyme

IKBA, IκB α, NFKBIA, Ikβ-α, IkB-alpha

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Galerie der Validierungsdaten

• 

  WB analysis using 10268-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10268-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB Alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10268-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10268-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB Alpha antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 10268-1-AP

  pig liver tissue were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB alpha antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10268-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10268-1-AP (IkB alpha antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10268-1-AP

  IP result of anti-IkB Alpha (IP:10268-1-AP, 4ug; Detection:10268-1-AP 1:15000) with HeLa cells lysate 1320 ug.

• 

  IHC staining of human stomach using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10268-1-AP (IkB alpha antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse kidney using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10268-1-AP (IkB alpha antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse kidney using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10268-1-AP (IkB Alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10268-1-AP (IkB alpha antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 10268-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10268-1-AP (IkB alpha antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of A431 using 10268-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using IkB Alpha antibody (10268-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10268-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IkB Alpha antibody (10268-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HeLa using 10268-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human IkB Alpha (10268-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, Hausschwein-Lebergewebe, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, HL-60-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe, humanes Nierengewebe, humanes Prostatakarzinomgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 458 publications below
• IHC: See 14 publications below
• IF: See 7 publications below
• FC: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

10268-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA IkB Alpha und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IkB Alpha fusion protein Ag0345
• Vollständiger Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC002601
• Gene symbol: IkB Alpha
• Gene ID (NCBI): 4792
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

Publikationen

Species	Application	Title
human	IF	Nat Cell Biol; Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.; Authors - Li Wang
human	WB	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu
mouse	WB	Mol Cell; Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation; Authors - Chuanlong Wang
human	WB,IF	Nucleic Acids Res; p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway; Authors - Xinxing Zhu
mouse	WB	Sci Adv; Elevated type I interferon responses potentiate metabolic dysfunction, inflammation, and accelerated aging in mtDNA mutator mice.; Authors - Yuanjiu Lei
human	WB	Genes Dev; PMID: 30463905; Authors - Rosa M Rubio

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

SONAM (Verified Customer) (10-04-2023)

Antibody worked well. No nonspecific band was observed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cancer cells

FH

CHAO (Verified Customer) (01-30-2020)

Weak bands for the heart lysates at basal state.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C

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14220-1-AP

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15649-1-AP

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12660-1-AP