NFKB2,p100 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NFKB2,p100 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

20

Kat-Nr. : 10409-2-AP

Synonyme

NFKB2, NF kappa B, LYT10, LYT 10, H2TF1

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Galerie der Validierungsdaten

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  WB analysis using 10409-2-AP

  WB result of 10409-2-AP (NFKB2,p100 antibody).

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  WB analysis of HeLa using 10409-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10409-2-AP (NFKB2,p100 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 10409-2-AP

  IP result of anti-NFKB2,p100 (IP:10409-2-AP, 4ug; Detection:10409-2-AP 1:600) with K-562 cells lysate 1200ug.

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  IHC staining of human liver cancer using 10409-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10409-2-AP (NFKB2,p100 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 10409-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10409-2-AP (NFKB2,p100 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 10409-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10409-2-AP (NFKB2,p100 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 10409-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10409-2-AP (NFKB2,p100 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of U2OS using 10409-2-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NFKB2 antibody (10409-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 8 publications below
• IF: See 2 publications below

Produktinformation

10409-2-AP bindet in WB, IHC, IF/ICC, IP, ELISA NFKB2,p100 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NFKB2,p100 fusion protein Ag0662
• Vollständiger Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC002844
• Gene symbol: NFKB2
• Gene ID (NCBI): 4791
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. This antibody can bind p100 isoform of NFKB2 spefically, but not p52 isoform.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; A proteomics landscape of circadian clock in mouse liver.; Authors - Yunzhi Wang
human	WB	Nat Commun; A translational program that suppresses metabolism to shield the genome.; Authors - Nathan C Balukoff
mouse	WB	J Hazard Mater; TiO2 nanosheets promote the transformation of vascular smooth muscle cells into foam cells in vitro and in vivo through the up-regulation of nuclear factor kappa B subunit 2.; Authors - Shuang Li
human	WB	PLoS Pathog; SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.; Authors - Muyu Xu
human	WB,IHC	Biochim Biophys Acta Mol Basis Dis; LRRC8A as a central mediator promotes colon cancer metastasis by regulating PIP5K1B/PIP2 pathway; Authors - Haifeng Zhang
human	WB	Cell Death Dis; Identification of 11(13)-dehydroivaxillin as a potent therapeutic agent against non-Hodgkin's lymphoma.; Authors - Xinhua Xiao