NFKB1,p105,p50 Polyklonaler Antikörper
NFKB1,p105,p50 Polyklonal Antikörper für FC, IF, IP, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Ratte und mehr (6)
Anwendung
WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 14220-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | A431-Zellen, HeLa-Zellen, Jurkat-Zellen, Raji-Zellen, U-87 MG-Zellen |
| Erfolgreiche IP | Jurkat-Zellen |
| Erfolgreiche Detektion in IF | HeLa-Zellen, HepG2-Zellen |
| Erfolgreiche Detektion in FC | HepG2-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunfluoreszenz (IF) | IF : 1:200-1:800 |
| Durchflusszytometrie (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 139 publications below |
| IHC | See 12 publications below |
| IF | See 22 publications below |
| IP | See 4 publications below |
| CoIP | See 1 publications below |
| ChIP | See 2 publications below |
| RIP | See 1 publications below |
Produktinformation
14220-1-AP bindet in WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA NFKB1,p105,p50 und zeigt Reaktivität mit human, Ratten
| Getestete Reaktivität | human, Ratte |
| In Publikationen genannte Reaktivität | human, Affe, Hausschwein, Huhn, Hund, Maus, Ratte, Rind |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | NFKB1,p105,p50 fusion protein Ag5458 |
| Vollständiger Name | nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 |
| Berechnetes Molekulargewicht | 105 kDa |
| Beobachtetes Molekulargewicht | 50 kDa, 105 kDa |
| GenBank-Zugangsnummer | BC051765 |
| Gene symbol | NFKB1 |
| Gene ID (NCBI) | 4790 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
NFkB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFkB is activated by various intra- and extracellular stimuli such as cytokines, oxidant free radicals, ultraviolet irradiation, and bacterial or viral products. NFkB is a family of transcription factors that consists of homo- and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL1 alpha and TNF alpha as mediated by IkB kinase and p38 MAPK. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. This antibody can bind both p105 and p50 isoforms of NFKB1.
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for NFKB1,p105,p50 antibody 14220-1-AP | Protokoll herunterladen |
| IF protocol for NFKB1,p105,p50 antibody 14220-1-AP | Protokoll herunterladen |
| IP protocol for NFKB1,p105,p50 antibody 14220-1-AP | Protokoll herunterladen |
| FC protocol for NFKB1,p105,p50 antibody 14220-1-AP | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Nat Immunol NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death. | ||
Nucleic Acids Res p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway | ||
Nat Microbiol Chloroquine ameliorates carbon tetrachloride-induced acute liver injury in mice via the concomitant inhibition of inflammation and induction of apoptosis | ||
Biomaterials A multifunctional anti-inflammatory drug that can specifically target activated macrophages, massively deplete intracellular H2O2, and produce large amounts CO for a highly efficient treatment of osteoarthritis. | ||
EMBO J The p53-inducible long noncoding RNA TRINGS protects cancer cells from necrosis under glucose starvation. | ||
Theranostics Prussian blue nanozyme-mediated nanoscavenger ameliorates acute pancreatitis via inhibiting TLRs/NF-κB signaling pathway. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ane (Verified Customer) (09-18-2023) | This antibody was used to detect p50 at different cell compartments and worked fine to dectect both phosphorilated and unphosphorilated forms of p50 in all cell compartments
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