NFIL3 Polyklonaler Antikörper

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• KD/KO Validated

NFIL3 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 11773-1-AP

Synonyme

NFIL3A, Interleukin-3-binding protein 1, Interleukin-3 promoter transcriptional activator, IL3BP1, E4BP4

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Galerie der Validierungsdaten

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  WB analysis using 11773-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11773-1-AP (NFIL3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11773-1-AP

  IP result of anti-NFIL3 (IP:11773-1-AP, 4ug; Detection:11773-1-AP 1:1000) with HeLa cells lysate 1600ug.

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  IHC staining of mouse stomach using 11773-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 11773-1-AP (NFIL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat stomach using 11773-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 11773-1-AP (NFIL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 11773-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 11773-1-AP (NFIL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11773-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 11773-1-AP (NFIL3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of NK92 using 11773-1-AP

  1X10^6 NK92 were intracellularly stained with 0.4 ug Anti-Human NFIL3 (11773-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, K562-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maus-Magengewebe, humanes Hautkrebsgewebe, Ratten-Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): NK92, NK92 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• ChIP: See 5 publications below

Produktinformation

11773-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA NFIL3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NFIL3 fusion protein Ag2380
• Vollständiger Name: nuclear factor, interleukin 3 regulated
• Berechnetes Molekulargewicht: 462 aa, 51 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC008197
• Gene symbol: NFIL3
• Gene ID (NCBI): 4783
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NFIL3, also named as E4BP4 and IL3BP1, belongs to the bZIP family and NFIL3 subfamily. It represses transcription from promoters with activating transcription factor (ATF) sites. NFIL3 activates transcription from the interleukin-3 promoter in T-cells. It competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). It is a component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. NFIL3 protects pro-B cells from programmed cell death. It is a key regulator of TH2 responses(PMID:21499227). The calculated molecular weight of NFIL3 is 51 kDa, but the modified NFIL3 is about 55-60 kDa.

Publikationen

Species	Application	Title
mouse	IF	Brain; Mislocated FUS is sufficient for gain-of-toxic-function amyotrophic lateral sclerosis phenotypes in mice.; Authors - Gen Shiihashi
mouse		Theranostics; The Circadian Clock Gene Bmal1 Controls Intestinal Exporter MRP2 and Drug Disposition.; Authors - Fangjun Yu
rat	WB	Neuropharmacology; Retigabine ameliorates acute stress-induced impairment of spatial memory retrieval through regulating USP2 signaling pathways in hippocampal CA1 area.; Authors - Cai Li
mouse	WB	Biochem Pharmacol; Tangeretin prevents cognitive deficit in delirium through activating RORα/γ-E4BP4 axis in mice; Authors - Min Chen
human	WB, ChIP	Front Med (Lausanne); NFIL3 Facilitates Neutrophil Autophagy, Neutrophil Extracellular Trap Formation and Inflammation During Gout via REDD1-Dependent mTOR Inactivation.; Authors - Honghu Tang
mouse	WB,chIP	Drug Metab Dispos; The Molecular Mechanism Regulating Diurnal Rhythm of Flavin-Containing Monooxygenase 5 in Mouse Liver.; Authors - Min Chen ; KD Validated