NFIB Polyklonaler Antikörper

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• KD/KO Validated

NFIB Polyklonal Antikörper für FC, IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29898-1-AP

Synonyme

CTF, HMGIC/NFIB, NF I/B, NF1 B, NFI B, NFI RED, NFIB, NFIB2, NFIB3, Nuclear factor 1 B type, Nuclear factor 1/B, nuclear factor I/B, TGGCA binding protein

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Galerie der Validierungsdaten

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  WB analysis using 29898-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29898-1-AP (NFIB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 29898-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NFIB antibody (29898-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  FC experiment of HeLa using 29898-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human NFIB (29898-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, HeLa-Zellen, HepG2-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

29898-1-AP bindet in WB, IF, FC, ELISA NFIB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NFIB fusion protein Ag31946
• Vollständiger Name: nuclear factor I/B
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 45-65 kDa
• GenBank-Zugangsnummer: BC001283
• Gene symbol: NFIB
• Gene ID (NCBI): 4781
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NFIB, also named as Nuclear factor 1, is a 420 amino acid protein, which belongs to the CTF/NF-I family and may bind DNA as a homodimer. NFIB recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. NFIB also exists in multiple isoforms in prostate cancer cells, with average molecular weights of 62 kDa, 57 kDa, and 49 kDa (PMID: 32692871).