NEUROD1 Polyklonaler Antikörper

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• KD/KO Validated

NEUROD1 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Rezensionen (1)

Kat-Nr. : 12081-1-AP

Synonyme

NEUROD, Class A basic helix-loop-helix protein 3, bHLHa3

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Galerie der Validierungsdaten

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  WB analysis using 12081-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12081-1-AP (NEUROD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Y79 using 12081-1-AP

  IP result of anti-NEUROD1 (IP:12081-1-AP, 3ug; Detection:12081-1-AP 1:200) with Y79 cells lysate 2000ug.

• 

  IHC staining of rat brain using 12081-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12081-1-AP (NEUROD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12081-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12081-1-AP (NEUROD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12081-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12081-1-AP (NEUROD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 12081-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12081-1-AP (NEUROD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12081-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12081-1-AP (NEUROD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12081-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12081-1-AP (NEUROD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 12081-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12081-1-AP (NEUROD1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 12081-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12081-1-AP (NEUROD1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen, Maus-Pankreasgewebe, Ratten-Pankreasgewebe
• Erfolgreiche IP: Y79-Zellen
• Erfolgreiche Detektion in IHC: Rattenhirngewebe, humanes Pankreaskarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Produktinformation

12081-1-AP bindet in WB, IHC, IF-P, IP, ELISA NEUROD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NEUROD1 fusion protein Ag2713
• Vollständiger Name: neurogenic differentiation 1
• Berechnetes Molekulargewicht: 356 aa, 40 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC009046
• Gene symbol: NEUROD1
• Gene ID (NCBI): 4760
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NeuroD is a member of the basic helix-loop-helix (bHLH) family of transcription factors. The basic helix-loop-helix (bHLH) proteins are transcription factors that are required for several aspects of development, including cell type determination, terminal differentiation and sex determination. Members of the myogenic determination family, MyoD, myf5, myogenin and MRF4, all have bHLH domains.These proteins function by forming heterodimers with E-proteins and binding to the canonical E-box sequence CANNTG. Neuro D is expressed transiently in a subset of neurons in the central and peripheral nervous systems at the time of their terminal differentiation into mature neurons. Moreover, ectopic expression of Neuro D in Xenopus embryos induces premature differentiation of neuronal precursors and Neuro D can convert presumptive epidermal cells into neurons.The lack of NeuroD in the brain results in severe defects in development. Human mutations have been linked to a number of types of diabetes including type I diabetes mellitus and maturity-onset diabetes of the young. The calculated molecular weight of NEUROD1 is 39 kDa, but the modified NEUROD1 protein is about 45-50 kDa.

Publikationen

Species	Application	Title
human	WB	Sci Adv; Prototypical oncogene family Myc defines unappreciated distinct lineage states of small cell lung cancer.; Authors - Ayushi S Patel
mouse	IF	Sci Adv; Neurons burdened by DNA double-strand breaks incite microglia activation through antiviral-like signaling in neurodegeneration; Authors - Gwyneth M Welch
mouse	WB	Biomaterials; Three-dimensional cell-culture platform based on hydrogel with tunable microenvironmental properties to improve insulin-secreting function of MIN6 cells.; Authors - Miao Zhang
mouse	WB	Proc Natl Acad Sci U S A; CHD8 dosage regulates transcription in pluripotency and early murine neural differentiation.; Authors - Sabina Sood
mouse	IHC	Cell Rep; PMID: 31189116; Authors - Franziska Böttger
mouse	IF	PLoS Biol; Centrioles are amplified in cycling progenitors of olfactory sensory neurons.; Authors - Kaitlin Ching

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain