NEU4 Polyklonaler Antikörper

NEU4 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 12995-1-AP

Synonyme

N acetyl alpha neuraminidase 4, NEU4, sialidase 4

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Galerie der Validierungsdaten

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  WB analysis using 12995-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12995-1-AP (NEU4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 12995-1-AP

  IP result of anti-NEU4 (IP:12995-1-AP, 4ug; Detection:12995-1-AP 1:500) with L02 cells lysate 1400ug.

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  IHC staining of human placenta using 12995-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12995-1-AP (NEU4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 12995-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12995-1-AP (NEU4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HepG2-Zellen, L02-Zellen
• Erfolgreiche IP: L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Produktinformation

12995-1-AP bindet in WB, IP, IF, IHC, ELISA NEU4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NEU4 fusion protein Ag3657
• Vollständiger Name: sialidase 4
• Berechnetes Molekulargewicht: 496 aa, 53 kDa
• Beobachtetes Molekulargewicht: 52-55 kDa
• GenBank-Zugangsnummer: BC012899
• Gene symbol: NEU4
• Gene ID (NCBI): 129807
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NEU4, also named as LP5125, belongs to a family of glycohydrolytic enzymes, which remove terminal sialic acid residues from various sialo derivatives, such as glycoproteins, glycolipids, oligosaccharides, and gangliosides. It may function in lysosomal catabolism of sialylated glycoconjugates. NEU4 has a broad substrate specificity being active on glycoproteins, oligosaccharides and sialylated glycolipids.

Publikationen

Species	Application	Title
human	WB	J Biol Chem; Macrophages discriminate glycosylation patterns of apoptotic cell-derived microparticles.; Authors - Bilyy Rostyslav O RO
mouse	WB,IP	Cell Signal; A novel epidermal growth factor receptor-signaling platform and its targeted translation in pancreatic cancer.; Authors - Gilmour Alanna M AM
rat	WB,IP	Cell Signal; PMID: 24583283; Authors - Farah Alghamdi
rat	WB,IF	Cell Signal; Neu1 sialidase and matrix metalloproteinase-9 cross-talk is essential for neurotrophin activation of Trk receptors and cellular signaling.; Authors - Jayanth Preethi P
mouse	WB,IF	Glycoconj J; Dependence of pathogen molecule-induced toll-like receptor activation and cell function on Neu1 sialidase.; Authors - Amith Schammim Ray SR
human	WB	Glycoconj J; Thymoquinone from nutraceutical black cumin oil activates Neu4 sialidase in live macrophage, dendritic, and normal and type I sialidosis human fibroblast cells via GPCR Galphai proteins and matrix metalloproteinase-9.; Authors - Finlay Trisha M TM