GNAS Polyklonaler Antikörper

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• KD/KO Validated

GNAS Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Rezensionen (1)

Kat-Nr. : 10150-2-AP

Synonyme

Neuroendocrine secretory protein 55, NESP55, LHAL tetrapeptide, GPIPIRRH peptide, GNAS1

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Galerie der Validierungsdaten

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  WB analysis using 10150-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10150-2-AP (GNAS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10150-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10150-2-AP (GNAS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10150-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10150-2-AP (GNAS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 10150-2-AP

  IP result of anti-GNAS (IP:10150-2-AP, 4ug; Detection:10150-2-AP 1:300) with mouse liver tissue lysate 4000ug.

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  IHC staining of mouse brain using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10150-2-AP (GNAS antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10150-2-AP (GNAS antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 10150-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GNAS antibody (10150-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 594-phalloidine (red).

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  IF Staining of MCF-7 using 10150-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using GNAS antibody (10150-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HepG2-Zellen, Maushirngewebe, Mauslebergewebe, MCF-7-Zellen, Rattenhirngewebe, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Kolongewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

10150-2-AP bindet in WB, IHC, IF/ICC, IP, ELISA GNAS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GNAS fusion protein Ag0197
• Vollständiger Name: GNAS complex locus
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC002722
• Gene symbol: GNAS
• Gene ID (NCBI): 2778
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.

Publikationen

Species	Application	Title
mouse	WB	Elife; Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.; Authors - Dao-Lai Zhang
human	WB	Cell Mol Life Sci; HDAC2 and TXNL1 distinguish aneuploid from diploid colorectal cancers.; Authors - Gemoll Timo T
human	WB,IF	Cell Mol Biol Lett; GNAS promotes inflammation-related hepatocellular carcinoma progression by promoting STAT3 activation.; Authors - Hongda Ding
mouse	WB,IF	Am J Physiol Renal Physiol; Ablation of Gsa impairs renal tubule proliferation after injury via CDK2/cyclin E.; Authors - Lele Liu ; KD Validated
human	WB	Tumour Biol; A novel GNAS-mutated human induced pluripotent stem cell model for understanding GNAS-mutated tumors.; Authors - Katsuhito Watanabe ; KO Validated
human,mouse	WB	Nat Commun; Activation of melanocortin-1 receptor signaling in melanoma cells impairs T cell infiltration to dampen antitumor immunity; Authors - Yazhong Cui

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Parijat (Verified Customer) (10-16-2023)

Works well.

• Applications: Western Blot