NES Polyklonaler Antikörper

NES Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30760-1-AP

Synonyme

FLJ21841, Nbla00170, NES, nestin

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Galerie der Validierungsdaten

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  WB analysis using 30760-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30760-1-AP (NES antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 30760-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30760-1-AP (NES antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 30760-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30760-1-AP (NES antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 30760-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30760-1-AP (NES antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 30760-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30760-1-AP (NES antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SGC-7901-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30760-1-AP bindet in WB, IHC, ELISA NES und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NES fusion protein Ag29600
• Vollständiger Name: nestin
• Berechnetes Molekulargewicht: 177 kDa
• Beobachtetes Molekulargewicht: 240-280 kDa
• GenBank-Zugangsnummer: NM_006617
• Gene symbol: NES
• Gene ID (NCBI): 10763
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. Nestin can be detected as 240-280 kDa. Studies point toward nestin heavy posttranslational modifications as possibly serving particular functions in podocytes and in a few other particular cell types(PMID: 17210924).