NEK6 Polyklonaler Antikörper

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• KD/KO Validated

NEK6 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 10378-1-AP

Synonyme

Protein kinase SID6-1512, Protein kinase SID6 1512, NimA-related protein kinase 6, NimA related protein kinase 6, Never in mitosis A-related kinase 6

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Galerie der Validierungsdaten

• 

  WB analysis using 10378-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10378-1-AP (NEK6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10378-1-AP

  WB result of NEK6 antibody (10378-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEK6 transfected HEK-293 cells.

• 

  WB analysis of HT-1080 using 10378-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 10378-1-AP (NEK6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10378-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10378-1-AP (NEK6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10378-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10378-1-AP (NEK6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10378-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10378-1-AP (NEK6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 10378-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 10378-1-AP (NEK6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 10378-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10378-1-AP (NEK6 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HT-1080.Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

10378-1-AP bindet in WB, IF/ICC, ELISA NEK6 und zeigt Reaktivität mit Affe, human, Maus, Ratten

• Getestete Reaktivität: Affe, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NEK6 fusion protein Ag0266
• Vollständiger Name: NIMA (never in mitosis gene a)-related kinase 6
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 35-45 kDa
• GenBank-Zugangsnummer: BC000101
• Gene symbol: NEK6
• Gene ID (NCBI): 10783
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Aspergillus nidulans 'never in mitosis A' (NIMA) is a serine/ threonine kinase that controls initiation of mitosis, whereas its inactivation is necessary for mitotic exit. NIMA-related kinases (NEKs) are a group of protein kinases that are homologous to NIMA. Evidence suggests that NEKs perform functions similar to those of NIMA. Human NIMA-related kinase 6 (NEK6, synonym: SID6-1512) is comprised of 338 amino acids and shows both nuclear and cytoplasmic localizations in HeLa cells. NEK6 is required for mitotic progression of human cells. NEK6 is phosphorylated and activated during M phase. Inhibition of Nek6 function by either overexpression of an inactive Nek6 mutant or elimination of endogenous Nek6 by siRNA arrests cells in M phase and triggers apoptosis. Recombinant human NEK6 protein produced in insect cells effectively phosphorylates histones H1 and H3, but not casein. Thus, these results suggest that, unlike other mammalian NIMA-related kinases, NEK6 is a mitotic histone kinase which regulates chromatin condensation in mammalian cells. NEK6 transcripts are ubiquitously expressed with the highest expression found in the heart and skeletal muscle, and the hNek6 gene is localized to human chromosome 9q33-34. NEK6 has four isoforms with MW 35-40 kDa.

Publikationen

Species	Application	Title
human	WB	Front Mol Biosci; LncRNA FAM13A-AS1 Promotes Renal Carcinoma Tumorigenesis Through Sponging miR-141-3p to Upregulate NEK6 Expression.; Authors - Xin Jun Wang
human	WB	J Oncol; MicroRNA-323a-3p Negatively Regulates NEK6 in Colon Adenocarcinoma Cells.; Authors - Zhongshi Hong
human	WB,IF	Int J Gen Med; NEK6 Promotes the Progression of Osteosarcoma Through Activating STAT3 Signaling Pathway by Down-Regulation of miR-26a-5p; Authors - Min Zhu
human	WB	Cancers (Basel); Differential Expression of NEK Kinase Family Members in Esophageal Adenocarcinoma and Barrett's Esophagus; Authors - Lei Chen