NEK2 Monoklonaler Antikörper

NEK2 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

3D7B4

Kat-Nr. : CL594-66632

Synonyme

HSPK 21, HsPK21, NEK2, NEK2A, NimA like protein kinase 1, NimA related protein kinase 2, NLK1

---

Galerie der Validierungsdaten

• 

  FC experiment of HeLa using CL594-66632

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human NEK2 (CL594-66632, Clone:3D7B4) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66632 bindet in FC (Intra) NEK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NEK2 fusion protein Ag21476
• Vollständiger Name: NIMA (never in mitosis gene a)-related kinase 2
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC043502
• Gene symbol: NEK2
• Gene ID (NCBI): 4751
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NEK2, also named as HSPK 21, belongs to the NEK family. NEK2 is well recognized as a multifunctional protein with roles in cell cycle regulation, such as centrosome duplication and separation, microtubule stabilization, kinetochore attachment and spindle assembly checkpoint. NEK2 is highly expressed in several cancer types, such as cholangiocarcinoma, breast, colorectal, and pancreatic cancer. NEK2 in mammals has three splice variants: NEK2A, NEK2B, and NEK2C. NEK2A is evenly distributed within the nuclei and cytoplasm, while NEK2B is mainly distributed in the cytoplasm, and NEK2C is mainly distributed in the nuclear region. As NEK2A, NEK2B and NEK2C exhibit overlapping or identical substrate usage, these variants are collectively referred to here as NEK2 (PMID:31092416).

Verwandte Produkte

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011