NEDD4L Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NEDD4L Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

36

Kat-Nr. : 13690-1-AP

Synonyme

NEDD4.2, KIAA0439, hNEDD4-2, HECT-type E3 ubiquitin transferase NED4L, EC:2.3.2.26

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Galerie der Validierungsdaten

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  WB analysis using 13690-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13690-1-AP (NEDD4L antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 13690-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 13690-1-AP (NEDD4L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 13690-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 13690-1-AP (NEDD4L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 13690-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13690-1-AP (NEDD4L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of C6 using 13690-1-AP

  IP result of anti-NEDD4L (IP:13690-1-AP, 4ug; Detection:13690-1-AP 1:300) with C6 cells lysate 1000 ug.

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  IHC staining of human prostate cancer using 13690-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13690-1-AP (NEDD4L Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human prostate cancer using 13690-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13690-1-AP (NEDD4L Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human prostate cancer using 13690-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13690-1-AP (NEDD4L Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human prostate cancer using 13690-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13690-1-AP (NEDD4L Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human stomach cancer using 13690-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13690-1-AP (NEDD4L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach cancer using 13690-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13690-1-AP (NEDD4L antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of PC-3 using 13690-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using NEDD4L antibody (13690-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C6-Zellen, HT-1080.Zellen, PC-12-Zellen, PC-3-Zellen
• Erfolgreiche IP: C6-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 29 publications below
• IHC: See 11 publications below
• IF: See 6 publications below
• IP: See 7 publications below
• CoIP: See 4 publications below

Produktinformation

13690-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA NEDD4L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NEDD4L fusion protein Ag4565
• Vollständiger Name: neural precursor cell expressed, developmentally down-regulated 4-like
• Berechnetes Molekulargewicht: 854 aa, 96 kDa
• Beobachtetes Molekulargewicht: 112 kDa, 125 kDa
• GenBank-Zugangsnummer: BC032597
• Gene symbol: NEDD4L
• Gene ID (NCBI): 23327
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NEDD4L(E3 ubiquitin-protein ligase NEDD4-like) is also named as KIAA0439, NEDL3, Nedd4-2. It accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substratesand and is the principal ubiquitin ligase that selectively targets activated SMAD2 and SMAD3 for destruction (PMID:19917253). Nedd4L binds the PY motif of ENaC COOH terminals and catalyzes ubiquitination of the NH2 terminus of the protein for subsequent degradation (PMID:18268134). It has some isoforms produced by alternative splicing. This antibody may have cross reaction with NEDD4 due to the high homology of the antigen.

Publikationen

Species	Application	Title
human	WB,IHC,IF	Nat Genet; Mutations in the HECT domain of NEDD4L lead to AKT-mTOR pathway deregulation and cause periventricular nodular heterotopia.; Authors - Loïc Broix
mouse	WB	J Exp Med; USP38 critically promotes asthmatic pathogenesis by stabilizing JunB protein.; Authors - Siyuan Chen
rat	WB	Cell Death Differ; Aagab acts as a novel regulator of NEDD4-1-mediated Pten nuclear translocation to promote neurological recovery following hypoxic-ischemic brain damage.; Authors - Chunfang Dai
human	WB	Theranostics; JP1 suppresses proliferation and metastasis of melanoma through MEK1/2 mediated NEDD4L-SP1-Integrin αvβ3 signaling.; Authors - Jiahua Cui
rat,human	CoIP,IF	Pharmacol Res; Piezo2 channel in nodose ganglia neurons is essential in controlling hypertension in a pathway regulated directly by Nedd4-2.; Authors - Lifang Huo
mouse	WB, IP, IF	Mol Cell Biol; Plk1 Phosphorylation of PTEN Causes a Tumor-Promoting Metabolic State.; Authors - Zhiguo Li