NDUFV3 Polyklonaler Antikörper

NDUFV3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 13430-1-AP

Synonyme

Renal carcinoma antigen NY-REN-4, NADH-ubiquinone oxidoreductase 9 kDa subunit, Complex I-9kD, Complex I 9kD, CI-9kD

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 13430-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13430-1-AP (NDUFV3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13430-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13430-1-AP (NDUFV3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13430-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13430-1-AP (NDUFV3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 13430-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13430-1-AP (NDUFV3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 13430-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13430-1-AP (NDUFV3 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 13430-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 13430-1-AP (NDUFV3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Herzgewebe, humanes Lebergewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

13430-1-AP bindet in WB, IHC, IF/ICC, ELISA NDUFV3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDUFV3 fusion protein Ag4234
• Vollständiger Name: NADH dehydrogenase (ubiquinone) flavoprotein 3, 10kDa
• Berechnetes Molekulargewicht: 108 aa, 12 kDa
• Beobachtetes Molekulargewicht: 10 kDa
• GenBank-Zugangsnummer: BC033766
• Gene symbol: NDUFV3
• Gene ID (NCBI): 4731
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDUFV3(NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial) is also named as CI-9kD,Renal carcinoma antigen NY-REN-4 and belongs to the complex I NDUFV3 subunit family.It is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.

Publikationen

Species	Application	Title
human	WB	J Proteome Res; Quantitative proteomics reveals oxygen-dependent changes in neuronal mitochondria affecting function and sensitivity to rotenone.; Authors - Villeneuve Lance L
human	WB	J Pharmacol Exp Ther; Metabolic Mechanisms and a Rational Combinational Application of Carboxyamidotriazole in Fighting Pancreatic Cancer Progression after Chemotherapy.; Authors - Rui Ju
human	WB	FEBS Lett; A novel isoform of the human mitochondrial complex I subunit NDUFV3.; Authors - Marris G Dibley
human	WB	F1000Res; Androgen-dependent alternative mRNA isoform expression in prostate cancer cells.; Authors - Jennifer Munkley
mouse	WB	Nat Struct Mol Biol; SCAF1 drives the compositional diversity of mammalian respirasomes; Authors - Irene Vercellino