NDUFV1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NDUFV1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

43

Kat-Nr. : 11238-1-AP

Synonyme

CI 51kD, Complex I 51kD, NDUFV1, UQOR1

---

Galerie der Validierungsdaten

• 

  WB analysis using 11238-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11238-1-AP (NDUFV1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11238-1-AP

  WB result of NDUFV1 antibody (11238-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDUFV1 transfected A549 cells.

• 

  IP experiment of A431 using 11238-1-AP

  IP result of anti-NDUFV1 (IP:11238-1-AP, 4ug; Detection:11238-1-AP 1:500) with A431 cells lysate 2000ug.

• 

  IHC staining of human lung cancer using 11238-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11238-1-AP (NDUFV1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 11238-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11238-1-AP (NDUFV1 Antibody) at dilution of 1:200 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Mausherzgewebe, U2OS-Zellen
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 42 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

11238-1-AP bindet in WB, IHC, IF, IP, ELISA NDUFV1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDUFV1 fusion protein Ag1764
• Vollständiger Name: NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC015645
• Gene symbol: NDUFV1
• Gene ID (NCBI): 4723
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DUFV1(NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial) is also named as UQOR1,Complex I-51kD and belongs to the complex I 51 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. It has 2 isoforms produced by alternative splicing. Defects in NDUFV1 are a cause of Leigh syndrome (LS) and mitochondrial complex I deficiency (MT-C1D).

Publikationen

Species	Application	Title
human	WB	Nature; In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer.; Authors - Milica Momcilovic
human	WB	Nature; Accessory subunits are integral for assembly and function of human mitochondrial complex I.; Authors - David A Stroud
human	WB	Mol Cell; Sengers Syndrome-Associated Mitochondrial Acylglycerol Kinase Is a Subunit of the Human TIM22 Protein Import Complex.; Authors - Yilin Kang
human,mouse	WB	Nat Commun; A salvage pathway maintains highly functional respiratory complex I.; Authors - Karolina Szczepanowska
mouse	WB	Brain; CLPP deficiency ameliorates neurodegeneration caused by impaired mitochondrial protein synthesis.; Authors - Anastasia Rumyantseva
mouse	WB	Dev Cell; Regulation of reverse electron transfer at mitochondrial complex I by unconventional Notch action in cancer stem cells.; Authors - Rani Ojha