NDUFA4L2 Monoklonaler Antikörper

NDUFA4L2 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1G1H10

Rezensionen (2)

Kat-Nr. : 66050-1-Ig

Synonyme

NDUFA4L2, NUOMS

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 66050-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66050-1-Ig (NDUFA4L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66050-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66050-1-Ig (NDUFA4L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66050-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66050-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 66050-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 66050-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HUVEC using 66050-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using NDUFA4L2 antibody (66050-1-Ig, Clone: 1G1H10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of MCF-7 using 66050-1-Ig

  Immunofluorescent analysis of MCF-7 cells, using NDUFA4L2 antibody 66050-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

• 

  IF Staining of HeLa using 66050-1-Ig

  Immunofluorescent analysis of Hela cells, using NDUFA4L2 antibody 66050-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HUVEC-Zellen, HeLa-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:200-1:1000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66050-1-Ig bindet in WB, IHC, IF, ELISA NDUFA4L2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NDUFA4L2 fusion protein Ag9233
• Vollständiger Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4-like 2
• Berechnetes Molekulargewicht: 87 aa, 10 kDa
• Beobachtetes Molekulargewicht: 10 kDa
• GenBank-Zugangsnummer: BC011910
• Gene symbol: NDUFA4L2
• Gene ID (NCBI): 56901
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDUFA4L2, also named as NUOMS, is a HIF-1α target gene that localizes to the mitochondria. It downregulates oxygen consumption and Complex I activity in hypoxia. NDUFA4L2 is involved in hypoxic adaptation by decreasing mitochondrial ROS production.

Publikationen

Species	Application	Title
human	WB	Cancer Biol Ther; NDUFA4L2 reduces mitochondrial respiration resulting in defective lysosomal trafficking in clear cell renal cell carcinoma; Authors - Jaclyn M Kubala

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kristian (Verified Customer) (06-07-2021)

The cells were stained with anti-NDUFA4l2 (green), Phalloidin (purple), and DAPI (blue). The NDUFA4L2 is localized in the mitochondria of the RCC4 cells.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: RCC4

NDUFA4L2 Antibody Immunofluorescence validation (1:200 dilution) in RCC4 (Cat no:66050-1-Ig)

FH

Daniel (Verified Customer) (04-16-2019)

Specific band in hypoxic mouse kidney. Additional band for Ndufa4.Note: The upper part of the blot (>25 kDa) was stained for a hypoxic marker (Car9).

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse kidney, hypoxic (lane1), normoxic (lane2)

NDUFA4L2 Antibody Western Blot, validation (1:1000 dilution) in Mouse kidney, hypoxic (lane1), normoxic (lane2) (Cat no:66050-1-Ig)