NDUFA4L2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NDUFA4L2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

20

Rezensionen (2)

Kat-Nr. : 16480-1-AP

Synonyme

NDUFA4L2, NUOMS

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Galerie der Validierungsdaten

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  WB analysis using 16480-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16480-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16480-1-AP

  IP Result of anti-NDUFA4L2 (IP:16480-1-AP, 4ug; Detection:16480-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of human liver cancer using 16480-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16480-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16480-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 16480-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16480-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 16480-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16480-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16480-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16480-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16480-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16480-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFA4L2 antibody (16480-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HeLa-Zellen, HepG2-Zellen, HUVEC-Zellen, PC-3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lebergewebe, humanes Nierengewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 8 publications below
• IHC: See 8 publications below
• IF: See 11 publications below
• IP: See 1 publications below

Produktinformation

16480-1-AP bindet in WB, IP, IHC, IF, ELISA NDUFA4L2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDUFA4L2 fusion protein Ag9600
• Vollständiger Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4-like 2
• Berechnetes Molekulargewicht: 87 aa, 10 kDa
• Beobachtetes Molekulargewicht: 10 kDa
• GenBank-Zugangsnummer: BC011910
• Gene symbol: NDUFA4L2
• Gene ID (NCBI): 56901
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDUFA4L2(NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2), also named as NUOMS, is a HIF-1α target gene that localizes to the mitochondria and belongs to the complex I NDUFA4 subunit family. It downregulates oxygen consumption and Complex I activity in hypoxia. NDUFA4L2 is involved in hypoxic adaptation by decreasing mitochondrial ROS production.

Publikationen

Species	Application	Title
human	IF	Adv Sci (Weinh); Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Esophageal Squamous Cell Carcinoma; Authors - Yongxu Jia
human	WB	Cell Metab; Induction of the mitochondrial NDUFA4L2 protein by HIF-1α decreases oxygen consumption by inhibiting Complex I activity.; Authors - Daniel Tello ; KD Validated
human	WB,IHC,IF,IP	Ther Adv Med Oncol; NDUFA4L2 promotes trastuzumab resistance in HER2-positive breast cancer.; Authors - Yuan Yuan
mouse	IF	Sci Signal; Acute O2 sensing through HIF2α-dependent expression of atypical cytochrome oxidase subunits in arterial chemoreceptors.; Authors - Alejandro Moreno-Domínguez ; KO Validated
rat	WB	Basic Res Cardiol; Metabolic adaptation to chronic hypoxia in cardiac mitochondria.; Authors - Heather Lisa C LC
human,rat	WB	Nutrients; Diosgenin Inhibits ROS Generation by Modulating NOX4 and Mitochondrial Respiratory Chain and Suppresses Apoptosis in Diabetic Nephropathy; Authors - Yujie Zhong

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Josh (Verified Customer) (10-28-2021)

Clear, good signal at expected MW (10kDa) by western blot. Non specific bands at 50 and 70 kDa (and to a lesser extent, 15 and 100 kDa).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: clear cell renal cell carcinoma cell line RCC4

NDUFA4L2 Antibody Western Blot validation (1:1000 dilution) in clear cell renal cell carcinoma cell line RCC4 (Cat no:16480-1-AP)

FH

Daniel (Verified Customer) (09-30-2019)

Kidney cancer mouse model stained for Ndufa4l2 (HRP detection by DAB stain).

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse kidney (TRACK model)

NDUFA4L2 Antibody Immunohistochemistry, validation (1:200 dilution) in Mouse kidney (TRACK model) (Cat no:16480-1-AP)