NCK2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NCK2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 10206-1-AP

Synonyme

Cytoplasmic protein NCK2, GRB4, Nck 2, NCK adaptor protein 2, NCK2, NCKbeta

---

Galerie der Validierungsdaten

• 

  WB analysis of U-937 using 10206-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 10206-1-AP (NCK2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 10206-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 10206-1-AP (NCK2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 10206-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10206-1-AP (NCK2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human placenta using 10206-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10206-1-AP (NCK2 Antibody) at dilution of 1:200 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

10206-1-AP bindet in WB, IHC, ELISA NCK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NCK2 fusion protein Ag0262
• Vollständiger Name: NCK adaptor protein 2
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC000103
• Gene symbol: NCK2
• Gene ID (NCBI): 8440
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lots of the protein-protein interactions that are essential for eukaryotic intracellular signal transduction are mediated by protein binding modules including SH2, SH3, and LIM domains. NCK is a SH3- and SH2-containing adaptor protein implicated in coordinating various signaling pathways, including those of growth factor receptors and cell adhesion receptors. NCK-2 is a ubiquitously expressed and NCK-related adaptor protein comprising primarily three N-terminal SH3 domains and one C-terminal SH2 domain. NCK-2 interacts with PINCH, a LIM-only protein implicated in integrin-linked kinase signaling, and recognizes several key components of growth factor kinase-signaling pathways including EGF receptors, PDGF receptor, and IRS-1. NCK-2 may thus function as an adaptor protein connecting the growth factor receptor-signaling pathways with the integrin-signaling pathways. NCK-2 also interacts with focal adhesion kinase (FAK), a cytoplasmic protein tyrosine kinase critically involved in the cellular control of motility.

Publikationen

Species	Application	Title
mouse	WB	Evid Based Complement Alternat Med; Icariin Alleviates Glucocorticoid-Induced Osteoporosis through EphB4/Ephrin-B2 Axis.; Authors - Mi Huang
human	WB	Biochem Pharmacol; KLF4 suppresses anticancer effects of brusatol via transcriptional upregulating NCK2 expression in melanoma; Authors - Xiaodong Li ; KD Validated