NCAPH Monoklonaler Antikörper

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• KD/KO Validated

NCAPH Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

3D2F11

Kat-Nr. : 67655-1-Ig

Synonyme

Barren homolog protein 1, BRRN, BRRN1, CAP H, CAPH, Condensin complex subunit 2, HCAP H, KIAA0074, NCAPH, XCAP H homolog

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Galerie der Validierungsdaten

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  WB analysis using 67655-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67655-1-Ig (NCAPH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of HEK-293 using 67655-1-Ig

  WB result of NCAPH antibody (67655-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCAPH transfected HEK-293 cells.

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  WB analysis using 67655-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67655-1-Ig (NCAPH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67655-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67655-1-Ig (NCAPH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67655-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67655-1-Ig (NCAPH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver cancer using 67655-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using NCAPH antibody (67655-1-Ig, Clone: 3D2F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, HEK-293-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Leberkarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67655-1-Ig bindet in WB, IHC, IF, ELISA NCAPH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NCAPH fusion protein Ag27748
• Vollständiger Name: non-SMC condensin I complex, subunit H
• Berechnetes Molekulargewicht: 741 aa, 83 kDa
• Beobachtetes Molekulargewicht: 83-100 kDa
• GenBank-Zugangsnummer: BC024211
• Gene symbol: NCAPH
• Gene ID (NCBI): 23397
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Non-SMC condensin I complex subunit H (NCAPH) is one of the three non-SMC subunits in condensin I, which belongs to a recently defined superfamily of proteins termed kleisins. Another two non-SMC subunits, CAP-D2 and CAP-G, share a highly degenerate repeating motif known as HEAT repeat. Some studies show that each subunit is essential for viability and plays an important role in mitotic chromosome architecture and segregation. In recent years, researchers found that the high expression of NCAPH was associated with poor prognosis in patients with non-small cell lung cancer and prostate cancer. Downregulation of NCAPH inhibited the proliferation, migration, and invasion of several cancer cells significantly. Moreover, NCAPH was involved in the regulation of mature chromosome condensation and DNA damage. These data suggest that NCAPH may be a key carcinogen involved in the development and progression of human malignant tumors. ( PMID: 28300828, PMID: 33311486 )