NCAPH Polyklonaler Antikörper

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• KD/KO Validated

NCAPH Polyklonal Antikörper für WB, IHC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 11515-1-AP

Synonyme

CND2, Chromosome-associated protein H, CAPH, CAP H, BRRN1

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Galerie der Validierungsdaten

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  WB analysis using 11515-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11515-1-AP (NCAPH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11515-1-AP

  WB result of NCAPH antibody (11515-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCAPH transfected HEK-293 cells.

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  IP experiment of HeLa using 11515-1-AP

  IP result of anti-NCAPH (IP:11515-1-AP, 3ug; Detection:11515-1-AP 1:800) with HeLa cells lysate 3000ug.

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  IHC staining of human colon cancer using 11515-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11515-1-AP (NCAPH antibody) at dilution of 1:50 (under 10x lens).

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  FC experiment of HEK-293 using 11515-1-AP

  1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug NCAPH Polyclonal antibody (11515-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 8 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Produktinformation

11515-1-AP bindet in WB, IHC, IF, FC (Intra), IP, ELISA NCAPH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NCAPH fusion protein Ag2076
• Vollständiger Name: non-SMC condensin I complex, subunit H
• Berechnetes Molekulargewicht: 741 aa, 83 kDa
• Beobachtetes Molekulargewicht: 83-100 kDa
• GenBank-Zugangsnummer: BC024211
• Gene symbol: NCAPH
• Gene ID (NCBI): 23397
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Non-SMC condensin I complex subunit H (NCAPH) is one of the three non-SMC subunits in condensin I, which belongs to a recently defined superfamily of proteins termed kleisins. Another two non-SMC subunits, CAP-D2 and CAP-G, share a highly degenerate repeating motif known as HEAT repeat. Some studies show that each subunit is essential for viability and plays an important role in mitotic chromosome architecture and segregation. In recent years, researchers found that the high expression of NCAPH was associated with poor prognosis in patients with non-small cell lung cancer and prostate cancer. Downregulation of NCAPH inhibited the proliferation, migration, and invasion of several cancer cells significantly. Moreover, NCAPH was involved in the regulation of mature chromosome condensation and DNA damage. These data suggest that NCAPH may be a key carcinogen involved in the development and progression of human malignant tumors. ( PMID: 28300828, PMID: 33311486 )

Publikationen

Species	Application	Title
human	WB,IHC	Int J Mol Sci; OCT1 Is a Poor Prognostic Factor for Breast Cancer Patients and Promotes Cell Proliferation via Inducing NCAPH.; Authors - Takuya Ogura ; KD Validated
human	WB	Cell Death Dis; NCAPH plays important roles in human colon cancer.; Authors - Liang Yin ; KD Validated
human	WB,IHC	Cell Cycle; NCAPH promotes cell proliferation and inhibits cell apoptosis of bladder cancer cells through MEK/ERK signaling pathway.; Authors - Bo Li ; KD Validated
human	WB	J Cell Sci; Ki-67 and condensins support the integrity of mitotic chromosomes through distinct mechanisms.; Authors - Masatoshi Takagi
human	WB	Sci Rep; NCAPH is a prognostic biomarker and associated with immune infiltrates in lung adenocarcinoma.; Authors - Chao Li
human	WB,IHC	Mol Carcinog; Non-SMC condensin I complex subunit H enhances proliferation, migration, and invasion of hepatocellular carcinoma.; Authors - Chengjun Sun ; KD Validated