NCAM2 Polyklonaler Antikörper

NCAM2 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 13850-1-AP

Synonyme

NCAM21, NCAM-2, NCAM 2, N CAM 2

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Galerie der Validierungsdaten

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  WB analysis using 13850-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13850-1-AP (NCAM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13850-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13850-1-AP (NCAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13850-1-AP

  IP result of anti-NCAM2 (IP:13850-1-AP, 4ug; Detection:13850-1-AP 1:5000) with mouse brain tissue lysate 2100 ug.

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  IHC staining of mouse brain using 13850-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13850-1-AP (NCAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13850-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13850-1-AP (NCAM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13850-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13850-1-AP (NCAM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 13850-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13850-1-AP (NCAM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below

Produktinformation

13850-1-AP bindet in WB, IHC, IP, ELISA NCAM2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NCAM2 fusion protein Ag4821
• Vollständiger Name: neural cell adhesion molecule 2
• Berechnetes Molekulargewicht: 93 kDa
• Beobachtetes Molekulargewicht: 100-125 kDa
• GenBank-Zugangsnummer: BC052946
• Gene symbol: NCAM2
• Gene ID (NCBI): 4685
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mammalian NCAM family has two members, NCAM1 and NCAM2 (PMID: 9729879; 20439718). Neural cell adhesion molecule 2 (NCAM2, also known as NCAM21) is a single-pass type I membrane protein that is highly expressed in the brain, which may play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons or in neurodegenerative diseases such as Alzheimer's disease (PMID: 26611261). NCAM2 has two isoforms: NCAM2.1 bearing transmembrane and cytoplasmic domains and the shorter NCAM2.2 isoform, which is GPI-anchored (PMID: 9334170; 9221781). The molecular weight of NCAM2 is around 125 kDa and 93 kDa (PMID: 9334170; 3171685).

Publikationen

Species	Application	Title
mouse	IHC	Science; Breast cancer exploits neural signaling pathways for bone-to-meninges metastasis; Authors - Andrew E Whiteley
		J Comp Neurol; Illuminating the terminal nerve: Uncovering the link between GnRH-1 neuron and olfactory development; Authors - Enrico Amato
mouse	IHC	bioRxiv; Illuminating the Terminal Nerve: Uncovering the Link between GnRH-1 and Olfactory Development; Authors - Enrico Amato