NCALD Polyklonaler Antikörper

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• KD/KO Validated

NCALD Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 12925-1-AP

Synonyme

NCALD, neurocalcin delta, neurocalcin δ

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 12925-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12925-1-AP (NCALD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 12925-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12925-1-AP (NCALD Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12925-1-AP

  WB result of NCALD antibody (12925-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCALD transfected A549 cells.

• 

  WB analysis of human cerebellum using 12925-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 12925-1-AP (NCALD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 12925-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 12925-1-AP (NCALD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12925-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12925-1-AP (NCALD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12925-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12925-1-AP (NCALD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 12925-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12925-1-AP (NCALD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 12925-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12925-1-AP (NCALD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 12925-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12925-1-AP (NCALD antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 12925-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12925-1-AP (NCALD antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12925-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12925-1-AP (NCALD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12925-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12925-1-AP (NCALD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A549-Zellen, humanes Cerebellum-Gewebe, humanes Herzgewebe, humanes Hirngewebe, Maus-Cerebellum-Gewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe, humanes Hirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 6 publications below
• IF: See 2 publications below

Produktinformation

12925-1-AP bindet in WB, IF, IHC, ELISA NCALD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NCALD fusion protein Ag3614
• Vollständiger Name: neurocalcin delta
• Berechnetes Molekulargewicht: 193 aa, 22 kDa
• Beobachtetes Molekulargewicht: 18-22 kDa
• GenBank-Zugangsnummer: BC036098
• Gene symbol: NCALD
• Gene ID (NCBI): 83988
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NCALD belongs to the recoverin family. And it is a member of the EF-hand calcium-binding protein superfamily. NCALD may be involved in the calcium-dependent regulation of rhodopsin phosphorylation. It plays an important role in the regulation of the neuronal signal transduction process. NCALD is a likely candidate for conferring susceptibility to diabetic nephropathy.

Publikationen

Species	Application	Title
mouse	IHC	Genes Dev; Gsx2 controls region-specific activation of neural stem cells and injury-induced neurogenesis in the adult subventricular zone.; Authors - López-Juárez Alejandro A
mouse	WB	Am J Hum Genet; Neurocalcin Delta Suppression Protects against Spinal Muscular Atrophy in Humans and across Species by Restoring Impaired Endocytosis.; Authors - Markus Riessland ; KO Validated
human	WB,IHC	Oncotarget; Upregulation of long intergenic noncoding RNA 00673 promotes tumor proliferation via LSD1 interaction and repression of NCALD in non-small-cell lung cancer.; Authors - Xuefei Shi
human	WB,IHC	Int J Oncol; N6‑methyladenosine upregulates miR‑181d‑5p in exosomes derived from cancer‑associated fibroblasts to inhibit 5‑FU sensitivity by targeting NCALD in colorectal cancer.; Authors - Shengli Pan
human	WB	Front Neurol; Transcriptome and Proteome Analysis in LUHMES Cells Overexpressing Alpha-Synuclein.; Authors - Matthias Höllerhage
human	IHC,WB	Biomark Med; NCALD as a potential predictive biomarker for the efficacy of platinum-based chemotherapy in ovarian cancer; Authors - Sarah M Konrad