N4-acetylcytidine Monoklonaler Antikörper

N4-acetylcytidine Monoklonal Antikörper für Dot Blot, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

chemical compound und mehr (1)

Anwendung

RIP, Dot Blot, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

3C2C2

Kat-Nr. : 68498-1-Ig

Synonyme

C11H15N3O6, Ac-rC, Acetylcytidine, ACETYL CYTOSINE, Ac4C

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  ELISA experiment of N4-acetylcytidine using 68498-1-Ig

  Indirect ELISA and competitive ELISA results show that this antibody is specific to ac4C (N4-acetylcytidine). Indirect ELISA (blue curve, refer to top X-right Y axis) was performed by coating BSA conjugated ac4C at 50ng/well followed by blocking with 5% non fat milk. Serial diluted primary antibody was added to the plates and incubated at 37℃. HRP-goat anti-mouse was used for detection. Competitive ELISA was performed similarly except that different concentration of Ac4C or its structure analogue compounds was mixed in 20ng/mL primary antibody (red curve, refer to bottom X-left Y axis).

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  Dot Blot experiment of HeLa using 68498-1-Ig

  Total RNA was isolated from HeLa cell line and was dotted to NC membrane at different amount as indicated above the dots. The membrane was blocked with 5% milk and blotted with N4-acetylcytidine antibody 68498-1-Ig at 1:2000 followed by incubation of HRP-goat anti-mouse secondary antibody. Signal was developed by ECL substrate. A parallel dot blot was performed using Mouse IgG1 isotype control Monoclonal antibody 66360-1-Ig at the same dose.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in ELISA: N4-acetylcytidine
• Erfolgreiche Detektion in Dot Blot: RNA isolate HeLa cells

Empfohlene Verdünnung

• Enzyme-linked Immunosorbent Assay (ELISA): ELISA :
• DOT BLOT: DOT BLOT : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• RIP: See 1 publications below

Produktinformation

68498-1-Ig bindet in RIP, Dot Blot, ELISA N4-acetylcytidine und zeigt Reaktivität mit chemical compound

• Getestete Reaktivität: chemical compound
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Verbindung
• Vollständiger Name: N4-acetylcytidine
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

N4-Acetylcytidine, CasNo. 3768-18-1, is a modified nucleoside and endogenous urinary nucleoside product of the degradation of tRNA, 18s rRNA and mRNA. N4-Acetylcytidine is a biological marker for various cancers with elevated concentrations present in urine. N4-Acetylcytidine is also a partially protected cytidine and therefore can be used as a synthetic building block to prepare further derivatized nucleosides such as 2',3'-dideoxycytidine. NAT10 catalyzes the formation of N4-acetylcytidine (ac4C) modification on mRNAs, 18S rRNA and tRNAs.

Protocol for Dot Blot:
https://www.ptglab.com/protocol/68498-1-IgDotBlot.pdf

Publikationen

Species	Application	Title
human	RIP	Int J Biol Macromol; N-acetyltransferase 10 impedes EZH2/H3K27me3/GABARAP axis mediated autophagy and facilitates lung cancer tumorigenesis through enhancing SGK2 mRNA acetylation; Authors - Nan Xiao