Marcksl1 Polyklonaler Antikörper

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• KD/KO Validated

Marcksl1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 10002-2-AP

Synonyme

AL022768, AW215397, AW536807, D4Bc1, F52, MacMARCKS, Macs 2, Macs 3, Macs2, Macs3, MARCKS like 1, Marcksl1, Mlp, Mrp

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 10002-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10002-2-AP (Marcksl1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Produktinformation

10002-2-AP bindet in WB, IF, IHC, ELISA Marcksl1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: MARCKS-like 1
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC006757
• Gene symbol: Marcksl1
• Gene ID (NCBI): 17357
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MacMARCKS is a member of MARCKS family of PKC substrate. It is widely used in the signal transduction studies as an indictor of PKC activation. The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-45 kDa in different species on SDS PAGE. MacMARCKS functions in many aspects of cell physiology including integrin activation, cell spreading and phagocytosis. Its functions are though to associate with its ability in binding and regulating actin and microtubule cytoskeleton. Current evidence indicated that this protein also involved controls membrane fluidity and molecular mobility of membrane proteins

Publikationen

Species	Application	Title
mouse	IHC	J Exp Med; Development of intestinal M cells and follicle-associated epithelium is regulated by TRAF6-mediated NF-κB signaling.; Authors - Takashi Kanaya
human	WB,IHC	J Biol Chem; The long noncoding RNA lncZic2 drives the self-renewal of liver tumor-initiating cells via the protein kinase C substrates MARCKS and MARCKSL1.; Authors - Zhenzhen Chen ; KO Validated
human,mouse	WB,IHC	Mol Cell Biol; c-Jun N-terminal kinase phosphorylation of MARCKSL1 determines actin stability and migration in neurons and in cancer cells.; Authors - Bj?rkblom Benny B ; KD Validated
mouse	WB	PLoS One; Colonic miRNA Expression/Secretion, Regulated by Intestinal Epithelial PepT1, Plays an Important Role in Cell-to-Cell Communication during Colitis.; Authors - Saravanan Ayyadurai
human	WB,IF	Exp Cell Res; The regulation of MacMARCKS expression by integrin beta3.; Authors - van den Bout Iman I
human	IHC	Clin Exp Metastasis; Identification of estrogen-responsive genes involved in breast cancer metastases to the bone.; Authors - Wang Jun J