Marcks Polyklonaler Antikörper

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• KD/KO Validated

Marcks Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus und mehr (2)

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 10004-2-Ig

Synonyme

Macs, Marcks, PKCSL

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 10004-2-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10004-2-Ig (Marcks antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10004-2-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10004-2-Ig (Marcks antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse pancreas using 10004-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10004-2-Ig (Marcks antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 10004-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10004-2-Ig (Marcks antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 10004-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10004-2-Ig (Marcks Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse pancreas using 10004-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10004-2-Ig (Marcks Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maus-Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

10004-2-Ig bindet in WB, IP, IF, IHC, ELISA Marcks und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: myristoylated alanine rich protein kinase C substrate
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 88 kDa
• GenBank-Zugangsnummer: BC046601
• Gene symbol: Marcks
• Gene ID (NCBI): 17118
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MARCKS is a member of MARCKS family of PKC substrate and it is also a widely accepted indicator of PKC activation. It shows a ubiquitous tissue distribution. The calculated molecular weight of MARCKS is 39 kDa and its apparent molecular weights were found at 68 kDa to 90 kDa in different species. This protein is a known to regulated cytoskeleton remodeling and membrane recycling. The anti-MARCKS antibody is raised against purified MARCKS protein from mouse brain using a method similar to that previously reported for MacMARCKS

Publikationen

Species	Application	Title
bovine	WB	Proc Natl Acad Sci U S A; MARCKS protein mediates hydrogen peroxide regulation of endothelial permeability.; Authors - Jin Benjamin Y BY
mouse	IF	J Cell Sci; Dynamic adhesions and MARCKS in melanoma cells.; Authors - Estrada-Bernal Adriana A
	WB,IP	Carcinogenesis; GRB-7 facilitates HER-2/Neu-mediated signal transduction and tumor formation.; Authors - Bai Tao T
	WB,IHC	J Biol Chem; The long noncoding RNA lncZic2 drives the self-renewal of liver tumor-initiating cells via the protein kinase C substrates MARCKS and MARCKSL1.; Authors - Zhenzhen Chen ; KO Validated
mouse	WB,IHC	Endocrinology; Accelerated proliferation and differential global gene expression in pancreatic islets of five-week-old heterozygous Men1 mice: Men1 is a haploinsufficient suppressor.; Authors - Lejonklou Margareta H MH
	WB,IF	Mol Biol Cell; Myristoylated, alanine-rich C-kinase substrate phosphorylation regulates growth cone adhesion and pathfinding.; Authors - Gatlin Jesse C JC