MYST2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MYST2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 13751-1-AP

Synonyme

KAT7, KAT 7, Histone acetyltransferase KAT7, HBOa, HBO1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 13751-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13751-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13751-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 13751-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 13751-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13751-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13751-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 13751-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13751-1-AP

  IP result of anti-MYST2 (IP:13751-1-AP, 4ug; Detection:13751-1-AP 1:500) with HeLa cells lysate 2480ug.

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  IHC staining of human ovary tumor using 13751-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13751-1-AP (MYST2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 13751-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13751-1-AP (MYST2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 13751-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney using 13751-1-AP (MYST2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse kidney using 13751-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney using 13751-1-AP (MYST2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 13751-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13751-1-AP (MYST2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 13751-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13751-1-AP (MYST2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of NIH/3T3 using 13751-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 13751-1-AP (MYST2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hodengewebe, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, PC-3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Mammakarzinomgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

13751-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA MYST2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYST2 fusion protein Ag4693
• Vollständiger Name: MYST histone acetyltransferase 2
• Berechnetes Molekulargewicht: 611 aa, 71 kDa
• Beobachtetes Molekulargewicht: 83 kDa
• GenBank-Zugangsnummer: BC032640
• Gene symbol: MYST2
• Gene ID (NCBI): 11143
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYST2, also named as HBO1, HBOa MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2, belongs to the MYST (SAS/MOZ) family. It specifically represses AR mediated transcription. MYST2 is a candidate oncogene. It enhances the anchorage-independent growth of breast cancer cells.(PMID:19372580) MYST2 is a histone acetyltransferase (HAT) which could exert oncogenic function in breast cancer. It is an important downstream molecule of ERa, and ERK1/2 signaling pathway may involved in the expression of HBO1 increased by E2.

Publikationen

Species	Application	Title
human	WB	Cancer Cell; Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.; Authors - Ling Li ; KO Validated
human	WB,IF,IP	Autophagy; KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.; Authors - Guokai Yan
human	WB	Cancer Res; Hbo1 is a cyclin E/CDK2 substrate that enriches breast cancer stem-like cells.; Authors - Mylinh T Duong
human	WB	Mol Cell Proteomics; Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm).; Authors - Heguo Yu
human	WB	Mol Cell Proteomics; Regulation of acetylation restores proteolytic function of diseased myocardium in mouse and human.; Authors - Ding Wang
human	WB	Mol Cell Biol; HBO1 (KAT7) Does Not Have an Essential Role in Cell Proliferation, DNA Replication, or Histone 4 Acetylation in Human Cells.; Authors - Andrew J Kueh