MYL3 Polyklonaler Antikörper

MYL3 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 10913-1-AP

Synonyme

Ventricular myosin alkali light chain, Myosin light chain 3, Myosin light chain 1, slow-twitch muscle B/ventricular isoform, MYL 3, MLC-lV/sb

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Galerie der Validierungsdaten

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  WB analysis using 10913-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10913-1-AP (MYL3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10913-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10913-1-AP (MYL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10913-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10913-1-AP (MYL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 10913-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10913-1-AP (MYL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 10913-1-AP

  IP result of anti-MYL3 (IP:10913-1-AP, 3ug; Detection:10913-1-AP 1:7000) with mouse heart tissue lysate 4000ug.

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  IHC staining of human heart using 10913-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10913-1-AP (MYL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10913-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10913-1-AP (MYL3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10913-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10913-1-AP (MYL3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of C2C12 using 10913-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using MYL3 antibody (10913-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of C2C12 using 10913-1-AP

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human MYL3 (10913-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, humanes Herzgewebe, Maus-Skelettmuskelgewebe, Rattenherzgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: C2C12-Zellen
• Erfolgreiche Detektion in FC (Intra): C2C12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

10913-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA MYL3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYL3 fusion protein Ag1364
• Vollständiger Name: myosin, light chain 3, alkali; ventricular, skeletal, slow
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22-27 kDa
• GenBank-Zugangsnummer: BC009790
• Gene symbol: MYL3
• Gene ID (NCBI): 4634
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYL3, also named as MLC1v, is an essential light chain of myosin that is associated with muscle contraction. It is expressed in ventricular and slow skeletal muscle. MYL3 may serve as a target for caspase-3 in dying cardiomyocytes. Mutations of MYL3 gene cause hypertrophic cardiomyopathy. MYL3 has been identified as potential serum biomarker for drug induced myotoxicity. Great increase in MYL3 serum concentration has been observed in rats with cardiac and skeletal muscle injury. (PMID:21685905)

Publikationen

Species	Application	Title
human	WB	Nat Biotechnol; A pipeline that integrates the discovery and verification of plasma protein biomarkers reveals candidate markers for cardiovascular disease.; Authors - Addona Terri A TA
	WB	J Proteome Res; Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy; Authors - Yuan Lin
mouse,human	WB,IHC,IF	Nat Commun; MYL3 protects chondrocytes from senescence by inhibiting clathrin-mediated endocytosis and activating of Notch signaling; Authors - He Cao
mouse	WB	Nat Commun; Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis; Authors - Yesheng Fu

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C