MYL12B Polyklonaler Antikörper

MYL12B Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 10324-1-AP

Synonyme

MLC 2, MLC 2A, MLC B, MLC20, MRLC2, MYL12B, MYLC2B, SHUJUN 1

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Galerie der Validierungsdaten

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  WB analysis using 10324-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10324-1-AP (MYL12B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10324-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10324-1-AP (MYL12B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10324-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10324-1-AP (MYL12B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10324-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10324-1-AP (MYL12B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 10324-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10324-1-AP (MYL12B antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon cancer using 10324-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10324-1-AP (MYL12B antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 1 publications below

Produktinformation

10324-1-AP bindet in WB, IF, IHC, ELISA MYL12B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYL12B fusion protein Ag0398
• Vollständiger Name: myosin, light chain 12B, regulatory
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 18-20 kDa
• GenBank-Zugangsnummer: BC004994
• Gene symbol: MYL12B
• Gene ID (NCBI): 103910
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYL12B is a regulatory subunit of myosin and plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. There are two groups of residues on the MYL12B that are phosphorylated by distinct kinases and have contrasting effects on myosin II biophysical properties.Phosphorylation at Thr18/Ser19 essentially "activates" the myosin molecule to produce force. The second group of phosphorylated residues is at the N-terminus of the MYL12B at Ser1, Ser2 and Thr9 and causes inhibitory effect on myosin activity.(PMID: 22136066)

Publikationen

Species	Application	Title
human	WB	Mol Biol Cell; Multiple regulatory steps control mammalian nonmuscle myosin II assembly in live cells.; Authors - Breckenridge Mark T MT
human	WB	Biochemistry (Mosc); Western Blotting-Based Quantitative Measurement of Myosin II Regulatory Light Chain Phosphorylation in Small Amounts of Non-muscle Cells.; Authors - O A Kazakova
human	WB,IF	BMC Cell Biol; Analysis of the role of Ser1/Ser2/Thr9 phosphorylation on myosin II assembly and function in live cells.; Authors - Beach Jordan R JR
human	WB	J Pept Sci; Design of peptidase-resistant peptide inhibitors of myosin light chain kinase.; Authors - Asker Y Khapchaev
human	WB	Biochemistry (Mosc); Long-Term Experimental Hyperglycemia Does Not Impair Macrovascular Endothelial Barrier Integrity and Function in vitro; Authors - Asker Y Khapchaev