MYH7-specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MYH7-specific Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (4)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

47

Kat-Nr. : 22280-1-AP

Synonyme

beta MHC, CMD1S, CMH1, MPD1, MYH7, MYH7-specific, MyHC beta, MyHC slow, MYHCB, Myosin 7, Myosin heavy chain 7, SPMD, SPMM

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Galerie der Validierungsdaten

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  WB analysis of mouse heart using 22280-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 22280-1-AP (MYH7-specific antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 22280-1-AP

  IP Result of anti-MYH7-specific (IP:22280-1-AP, 5ug; Detection:22280-1-AP 1:300) with mouse heart tissue lysate 4000ug.

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  IHC staining of mouse heart using 22280-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22280-1-AP (MYH7-specific antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 22280-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22280-1-AP (MYH7-specific antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 22280-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22280-1-AP (MYH7-specific antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse heart using 22280-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 22280-1-AP (MYH7-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse heart using 22280-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using MYH7-specific antibody (22280-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse skeletal muscle using 22280-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 22280-1-AP (MYH7-specific antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mausherzgewebe, Maus-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:600-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 40 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Produktinformation

22280-1-AP bindet in WB, IP, IHC, IF, ELISA MYH7-specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Zebrafisch, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myosin, heavy chain 7, cardiac muscle, beta
• Berechnetes Molekulargewicht: 223 kDa
• Beobachtetes Molekulargewicht: 200-220 kDa
• GenBank-Zugangsnummer: NM_000257
• Gene symbol: MYH7
• Gene ID (NCBI): 4625
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Myosin is the major contractile protein of all eukaryotic cells, existing as a hexameric molecule composed of two myosin heavy chains (MHCs) that contain the catalytic sites for ATP hydrolysis, and four lower molecular weight myosin light chains. MYH7 encodes the slow/beta-cardiac myosin heavy chain (MHC), which is the major MHC isoform of cardiac ventricles. It is also expressed in slow, oxidative, type 1 muscle fibers of skeletal muscle. Numerous mutations of MYH7 have been linked to familial hypertrophic cardiomyopathy. This antibody can specifically recognize endogenous MYH7, not cross-reacting with other MHCs. (2523412, 14520662)

Publikationen

Species	Application	Title
human	WB	Science; HEART DISEASE. Titin mutations in iPS cells define sarcomere insufficiency as a cause of dilated cardiomyopathy.; Authors - John T Hinson
mouse	WB	Circulation; Hyperglycemia-Driven Inhibition of AMP-Activated Protein Kinase α2 Induces Diabetic Cardiomyopathy by Promoting Mitochondria-Associated Endoplasmic Reticulum Membranes In Vivo.; Authors - Shengnan Wu
		Dev Cell; Force Generation via β-Cardiac Myosin, Titin, and α-Actinin Drives Cardiac Sarcomere Assembly from Cell-Matrix Adhesions.; Authors - Anant Chopra
human	WB	Elife; Independent regulation of Z-lines and M-lines during sarcomere assembly in cardiac myocytes revealed by the automatic image analysis software sarcApp; Authors - Abigail C Neininger-Castro ; KD Validated
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Dectin-1 deficiency alleviates diabetic cardiomyopathy by attenuating macrophage-mediated inflammatory response; Authors - Na Yang
pig	WB	J Agric Food Chem; Dietary Taurine Supplementation Improves the Meat Quality, Muscle Fiber Type, and Mitochondrial Function of Finishing Pigs; Authors - Wenting An

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