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MVK Monoklonaler Antikörper

MVK Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F1D3

Kat-Nr. : CL488-67820

Synonyme

FLJ96772, LRBP, mevalonate kinase, MK, MVK, MVLK

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated MVK Monoclonal antib (CL488-67820)

    FC experiment of HepG2 using CL488-67820

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MVK (CL488-67820, Clone:2F1D3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in FCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67820 bindet in FC (Intra) MVK und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen MVK fusion protein Ag28887
Vollständiger Name mevalonate kinase
Berechnetes Molekulargewicht 396 aa, 42 kDa
Beobachtetes Molekulargewicht 42 kDa
GenBank-ZugangsnummerBC016140
Gene symbol MVK
Gene ID (NCBI) 4598
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MVK (Mevalonate kinase) is a 42 kDa cytoplasmic protein that belongs to the GHMP kinase family. Mevalonate kinase catalyzes the ATP-dependent phosphorylation of mevalonic acid to form mevalonate 5-phosphate. Defects in mevalonate kinase can cause mevalonic aciduria (MEVA). It is an accumulation of mevalonic acid which causes a variety of symptoms such as psychomotor retardation, dysmorphic features, cataracts, hepatosplenomegaly, lymphadenopathy, anemia, hypotonia, myopathy and ataxia.

Protokolle

Produktspezifische Protokolle
FC protocol for CL Plus 488 MVK antibody CL488-67820Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen