MTHFD2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MTHFD2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

41

Rezensionen (2)

Kat-Nr. : 12270-1-AP

Synonyme

Methenyltetrahydrofolate cyclohydrolase, Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial

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Galerie der Validierungsdaten

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  WB analysis using 12270-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12270-1-AP (MTHFD2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12270-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12270-1-AP (MTHFD2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 12270-1-AP

  IP result of anti-MTHFD2 (IP:12270-1-AP, 4ug; Detection:12270-1-AP 1:4000) with A549 cells lysate 1480 ug.

• 

  IP experiment of mouse testis using 12270-1-AP

  IP result of anti-MTHFD2 (IP:12270-1-AP, 4ug; Detection:12270-1-AP 1:300) with mouse testis tissue lysate 4000ug.

• 

  IHC staining of human colon cancer using 12270-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12270-1-AP (MTHFD2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12270-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12270-1-AP (MTHFD2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12270-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MTHFD2 antibody (12270-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HCT 116-Zellen, HEK-293T-Zellen, Jurkat-Zellen, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche IP: A549-Zellen, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 36 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

12270-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA MTHFD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MTHFD2 fusion protein Ag2911
• Vollständiger Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
• Berechnetes Molekulargewicht: 350 aa, 38 kDa
• Beobachtetes Molekulargewicht: 33-38 kDa
• GenBank-Zugangsnummer: BC017054
• Gene symbol: MTHFD2
• Gene ID (NCBI): 10797
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) is a folate-coupled mitochondrial metabolic enzyme characterized by methylenetetrahydrofolate dehydrogenase and cyclohydrolase activity. It was demonstrated that suppression of MTHFD2 inhibits methylation reactions, decreases protein synthesis and disrupts redox homeostasis, which may ultimately result in significant changes in cellular metabolic phenotype. MTHFD2 is reported to be up‐regulated in various tumours, such as lung adenocarcinoma(PMID: 34121323) and breast cancer(PMID: 34007312).

Publikationen

Species	Application	Title
human	WB	Nature; Oxidative stress inhibits distant metastasis by human melanoma cells.; Authors - Elena Piskounova
mouse	WB	Science; mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.; Authors - Issam Ben-Sahra
human	WB	Cancer Cell; Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming.; Authors - Yoichiro Mitsuishi
human	WB	Cell Metab; Epstein-Barr-Virus-Induced One-Carbon Metabolism Drives B Cell Transformation.; Authors - Liang Wei Wang
mouse	WB	Cell Metab; Perturbed Redox Signaling Exacerbates a Mitochondrial Myopathy.; Authors - Sukru Anil Dogan
mouse	WB	Cell Metab; mTORC1 Regulates Mitochondrial Integrated Stress Response and Mitochondrial Myopathy Progression.; Authors - Nahid A Khan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ayse Tarbin (Verified Customer) (03-16-2023)

It was a sample gift antibody. It has a band at corresponding kDa but there are also unspesific bands just bellow 30 kDa.

• Applications: Western Blot, Cell culture
• Primary Antibody Dilution: 1:2000 in 5% milk in TBST
• Cell Tissue Type: Mouse satellite cells

MTHFD2 Antibody Western Blot,Cell culture validation (1:2000 in 5% milk in TBST dilution) in Mouse satellite cells (Cat no:12270-1-AP)

FH

Hua (Verified Customer) (10-21-2020)

There is no band at the expected size. There is a strong non-specific band at 100 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mouse liver lysate

MTHFD2 Antibody Western Blot validation (1:500 dilution) in mouse liver lysate (Cat no:12270-1-AP)