MT3 Polyklonaler Antikörper

MT3 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 12179-1-AP

Synonyme

GIF, GIFB, GRIF, Growth inhibitory factor, metallothionein 3, Metallothionein III, MT 3, MT III, MT3

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Galerie der Validierungsdaten

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  IHC staining of mouse brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12179-1-AP (MT3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hirngewebe, humanes Hodengewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

12179-1-AP bindet in IHC, ELISA MT3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MT3 fusion protein Ag2823
• Vollständiger Name: metallothionein 3
• Berechnetes Molekulargewicht: 68 aa, 7 kDa
• GenBank-Zugangsnummer: BC013081
• Gene symbol: MT3
• Gene ID (NCBI): 4504
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MT3(Metallothionein-3) is also names as GIF(Growth inhibitory factor),GIFB inhibits N-glycosylation of IgE-binding factors and that unglycosylated IgE-binding factors selectively suppressed IgE synthesis.The major cell source of GIF is antigen-specific suppressor T(Ts)cell(PMID:10967025).The molecular size of GIF from T cells is 15 kDa, while GIF from macrophages consisted of 40-and 15-kDa molecules(PMID:2417235).

Publikationen

Species	Application	Title
pig		Int J Mol Sci; Integrating Transcriptomics and Proteomics to Characterize the Intestinal Responses to Cadmium Exposure Using a Piglet Model; Authors - Yikun Li