MRPL49 Polyklonaler Antikörper

MRPL49 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC,ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 15542-1-AP

Synonyme

C11orf4, L49mt, MRP L49, MRPL49, Neighbor of FAU, NOF, NOF1, Protein NOF1

---

Galerie der Validierungsdaten

• 

  WB analysis of BxPC-3 using 15542-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15542-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15542-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 15542-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15542-1-AP

  IP Result of anti-MRPL49 (IP:15542-1-AP, 4ug; Detection:15542-1-AP 1:500) with HeLa cells lysate 3360ug.

• 

  IHC staining of human breast cancer using 15542-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15542-1-AP (MRPL49 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15542-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15542-1-AP (MRPL49 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, A549-Zellen, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below

Produktinformation

15542-1-AP bindet in WB, IP, IHC,ELISA MRPL49 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MRPL49 fusion protein Ag7898
• Vollständiger Name: mitochondrial ribosomal protein L49
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 19 kDa
• GenBank-Zugangsnummer: BC004378
• Gene symbol: MRPL49
• Gene ID (NCBI): 740
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MRPL49, also named as C11orf4, NOF1, or SW-cl.67, is a 166 amino acid protein, which belongs to the ribosomal protein L49em family. MRPL49 localizes in the Mitochondrion and is widely expressed in various tissues. MRPL49 is a regulator of C3 convertase of classical pathway.

Publikationen

Species	Application	Title
human	WB	Mol Cell; Largen: a molecular regulator of mammalian cell size control.; Authors - Kazuo Yamamoto
human	WB	Nucleic Acids Res; C7orf30 specifically associates with the large subunit of the mitochondrial ribosome and is involved in translation.; Authors - Wanschers Bas F J BF
human	WB	Nucleic Acids Res; Replication factors transiently associate with mtDNA at the mitochondrial inner membrane to facilitate replication.; Authors - Nina Rajala
human	WB	Sci Rep; Human Mitochondrial DNA-Protein Complexes Attach to a Cholesterol-Rich Membrane Structure.; Authors - Joachim M Gerhold
mouse	WB	Cell Rep; MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome Composition.; Authors - Jakob D Busch
human	WB	Nucleic Acids Res; Mitochondrial RNA granules are critically dependent on mtDNA replication factors Twinkle and mtSSB.; Authors - Fenna Hensen