MRE11 Polyklonaler Antikörper

MRE11 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 10744-1-AP

Synonyme

MRE11A, MRE11 homolog A, MRE11 homolog 1, HNGS1, EC:3.1.-.-

---

Galerie der Validierungsdaten

• 

  WB analysis using 10744-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10744-1-AP (MRE11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10744-1-AP

  IP result of anti-MRE11 (IP:10744-1-AP, 4ug; Detection:10744-1-AP 1:600) with HeLa cells lysate 1200ug.

• 

  IHC staining of human lymphoma using 10744-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10744-1-AP (MRE11 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of A431 using 10744-1-AP

  Immunofluorescent analysis of A431 cells using 10744-1-AP (MRE11 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

10744-1-AP bindet in WB, IHC, IF, IP, ChIP, ELISA MRE11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MRE11 fusion protein Ag1105
• Vollständiger Name: MRE11 meiotic recombination 11 homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 81 kDa
• Beobachtetes Molekulargewicht: 81 kDa
• GenBank-Zugangsnummer: BC005241
• Gene symbol: MRE11A
• Gene ID (NCBI): 4361
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mre11 (meiotic recombination 11), also named as Mre11A, is a component of the MRN( MRE11/RAD50/NBS1) complex which is a versatile complex, playing a key role in the sensing, processing, and repair of DSBs. MRE11 possesses both endonuclease and 3'-5' exonuclease activity. It contains two DNA-binding domains (DBDs), enabling it to bind both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). After DSBs, MRE11 is loaded onto DSBs sites and cleaves DNA by cooperating with CtIP (CtBP [C terminal binding protein] interacting protein) to initiate end resection. It's reported that MRE11 is over-expressed in breast cancers. (PMID:38128537; 22914783)

Publikationen

Species	Application	Title
human	WB	Sci Transl Med; Guanosine diphosphate-mannose suppresses homologous recombination repair and potentiates antitumor immunity in triple-negative breast cancer; Authors - Jia-Han Ding
human	WB	Nat Commun; Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatin; Authors - Tao Zhang
human	WB,IF	Oncogene; TRIM24 is critical for the cellular response to DNA double-strand breaks through regulating the recruitment of MRN complex; Authors - Ya Wang
human	WB,IHC,ChIP	Cell Death Dis; Acquired temozolomide resistance in MGMTlow gliomas is associated with regulation of homologous recombination repair by ROCK2.; Authors - Xin Zhang
human	WB	Cell Mol Biol Lett; MRE11A: a novel negative regulator of human DNA mismatch repair; Authors - Demin Du
human	IF	Cell Genom; NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors; Authors - Yahui Wang