MOXD1 Polyklonaler Antikörper

MOXD1 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 11545-1-AP

Synonyme

PRO5780, MOX, monooxygenase, DBH like 1, Monooxygenase X, EC:1.14.17.-

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Galerie der Validierungsdaten

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  WB analysis using 11545-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11545-1-AP (MOXD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 11545-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11545-1-AP (MOXD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11545-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11545-1-AP (MOXD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 11545-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11545-1-AP (MOXD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 11545-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11545-1-AP (MOXD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 11545-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MOXD1 antibody (11545-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 11545-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MOXD1 antibody (11545-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

11545-1-AP bindet in WB, IHC, IF-P, ELISA MOXD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MOXD1 fusion protein Ag2060
• Vollständiger Name: monooxygenase, DBH-like 1
• Berechnetes Molekulargewicht: 69 kDa
• Beobachtetes Molekulargewicht: 63-70 kDa
• GenBank-Zugangsnummer: BC018756
• Gene symbol: MOXD1
• Gene ID (NCBI): 26002
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Monooxygenase DBH like 1 (MOXD1) is a member of the copper monooxygenase family, including dopamine monooxygenase (dopamine β-monooxygenase, DBM) and peptidylglycine α-hydroxylating monooxygenase (PHM). MOXD1 is expressed during the development of the neural crest, and distributed along the olfactory bulb, cerebellum, brain stem, and parietal cortex with the development of nerve cells, participating in the basal plate. MOXD1 is highly expressed in GBM cells and can promote the growth of cancer cells (PMID: 35393406). In addition, MOXD1 is a gate-keeper of organ homeostasis and functions as a tumor-suppressor in neuroblastoma

Publikationen

Species	Application	Title
mouse	WB	Funct Integr Genomics; Depletion of PIEZO1 expression is accompanied by upregulating p53 signaling in mice with perioperative neurocognitive disorder; Authors - Fuhai Bai
human,mouse	WB,IHC	Life Sci; Fenofibrate inhibits MOXD1 and PDZK1IP1 expression and improves lipid deposition and inflammation in mice with alcoholic fatty liver; Authors - Tongtong Pan