MMP20 Polyklonaler Antikörper

MMP20 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 55467-1-AP

Synonyme

Enamel metalloproteinase, Enamelysin, matrix metallopeptidase 20, Matrix metalloproteinase 20, MMP 20, MMP20

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 55467-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55467-1-AP (MMP20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 55467-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 55467-1-AP (MMP20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 55467-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 55467-1-AP (MMP20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 55467-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 55467-1-AP (MMP20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55467-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55467-1-AP (MMP20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 55467-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 55467-1-AP (MMP20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 55467-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 55467-1-AP (MMP20 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 55467-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 55467-1-AP (MMP20 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 55467-1-AP

  Immunofluorescent analysis of HeLa cells using 55467-1-AP (MMP20 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

55467-1-AP bindet in WB, IHC, IF, ELISA MMP20 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: matrix metallopeptidase 20
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: NM_004771
• Gene symbol: MMP20
• Gene ID (NCBI): 9313
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MMP20 belongs to the peptidase M10A family. It degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). MMP20 may play a central role in tooth enamel formation. Defects in MMP20 are the cause of amelogenesis imperfecta hypomaturation type 2A2 (AI2A2). This antibody is specific to MMP20.

Publikationen

Species	Application	Title
human	IF	Int J Mol Sci; Overexpression of miR-1306-5p, miR-3195, and miR-3914 Inhibits Ameloblast Differentiation through Suppression of Genes Associated with Human Amelogenesis Imperfecta.; Authors - Hiroki Yoshioka
mouse	WB	Front Genet; Crucial Roles of microRNA-16-5p and microRNA-27b-3p in Ameloblast Differentiation Through Regulation of Genes Associated With Amelogenesis Imperfecta.; Authors - Akiko Suzuki
rat	WB	Cell Prolif; Smurf1 regulates ameloblast polarization by ubiquitination-mediated degradation of RhoA; Authors - Haoman Niu