MMADHC Polyklonaler Antikörper

MMADHC Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC

Konjugation

Unkonjugiert

Kat-Nr. : 23191-1-AP

Synonyme

C2orf25, cblD, CL25022, MMADHC, My011

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Galerie der Validierungsdaten

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  WB analysis using 23191-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23191-1-AP (MMADHC antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23191-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23191-1-AP (MMADHC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 23191-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 23191-1-AP (MMADHC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 23191-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23191-1-AP (MMADHC Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 23191-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23191-1-AP (MMADHC Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas using 23191-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23191-1-AP (MMADHC Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 23191-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23191-1-AP (MMADHC Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 23191-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 23191-1-AP (MMADHC antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 23191-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 23191-1-AP (MMADHC antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 23191-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 23191-1-AP (MMADHC Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 23191-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 23191-1-AP (MMADHC Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, MCF-7-Zellen, NIH3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe, humanes Herzgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

23191-1-AP bindet in WB, IHC MMADHC und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MMADHC fusion protein Ag19343
• Vollständiger Name: methylmalonic aciduria (cobalamin deficiency) cblD type, with homocystinuria
• Berechnetes Molekulargewicht: 296 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC022859
• Gene symbol: MMADHC
• Gene ID (NCBI): 27249
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MMADHC is a mitochondrial protein that is involved in an early step of vitamin B12 metabolism. Vitamin B12 (cobalamin) is essential for normal development and survival in humans. MMADHC is a 296-amino acids (32.9 kDa) protein with an N-terminal disordered region (amino acids 1-107) containing a potential mitochondrial leader sequence (MLS; amino acids 1-12), and a C-terminal Nitro Reductase-like domain (NTR; amino acids 108-296). Mutations in this gene cause methylmalonic aciduria and homocystinuria type cblD (MMADHC), a disorder of cobalamin metabolism that is characterized by decreased levels of the coenzymes adenosylcobalamin and methylcobalamin.

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