MMAB Polyklonaler Antikörper

MMAB Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11137-1-AP

Synonyme

EC:2.5.1.-, Corrinoid adenosyltransferase MMAB, cblB, ATR, ATP:co(I)rrinoid adenosyltransferase MMAB

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 11137-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11137-1-AP (MMAB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 11137-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11137-1-AP (MMAB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11137-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11137-1-AP (MMAB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11137-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11137-1-AP (MMAB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 11137-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11137-1-AP (MMAB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11137-1-AP

  IP result of anti-MMAB (IP:11137-1-AP, 4ug; Detection:11137-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of human ovary tumor using 11137-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11137-1-AP (MMAB antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 11137-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MMAB antibody (11137-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, BxPC-3-Zellen, HepG2-Zellen, L02-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

11137-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA MMAB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MMAB fusion protein Ag1602
• Vollständiger Name: methylmalonic aciduria (cobalamin deficiency) cblB type
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC011831
• Gene symbol: MMAB
• Gene ID (NCBI): 326625
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The MMAB gene encodes cob(I)alamin adenosyltransferase, which catalyzes the final step in the synthesis of the coenzyme adenosylcobalamin (AdoCbl). AdoCbl is a vitamin B12-containing coenzyme for methylmalonyl-CoA mutase. The deduced 250-amino acid protein has a calculated molecular mass of 27.3 kD.

Publikationen

Species	Application	Title
mouse	WB	Hum Mol Genet; Pharmacological chaperones as a potential therapeutic option in methylmalonic aciduria cblB type.; Authors - Jorge-Finnigan Ana A
human	WB	Hum Mutat; Functional and structural analysis of five mutations identified in methylmalonic aciduria cblB type.; Authors - Jorge-Finnigan Ana A
human	WB,IF	Biochim Biophys Acta Mol Basis Dis; Hepatocyte-like cells differentiated from methylmalonic aciduria cblB type induced pluripotent stem cells: A platform for the evaluation of pharmacochaperoning.; Authors - Á Briso-Montiano
	WB	Biochim Biophys Acta; New perspectives for pharmacological chaperoning treatment in methylmalonic aciduria cblB type.; Authors - S Brasil