MLXIP Polyklonaler Antikörper

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• KD/KO Validated

MLXIP Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Kat-Nr. : 13614-1-AP

Synonyme

MLX-interacting protein, MLX interacting protein, MIR, KIAA0867, Class E basic helix-loop-helix protein 36

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 13614-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13614-1-AP (MLXIP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 13614-1-AP

  IP result of anti-MLXIP (IP:13614-1-AP, 4ug; Detection:13614-1-AP 1:300) with K-562 cells lysate 3200ug.

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  IP experiment of K-562 using 13614-1-AP

  IP result of anti-MLXIP (IP:13614-1-AP, 4ug; Detection:13614-1-AP 1:300) with K-562 cells lysate 3200ug.

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  IHC staining of human skeletal muscle using 13614-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 13614-1-AP (MLXIP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skeletal muscle using 13614-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 13614-1-AP (MLXIP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse skeletal muscle using 13614-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13614-1-AP (MLXIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 13614-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13614-1-AP (MLXIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 13614-1-AP

  Immunofluorescent analysis of HepG2 cells, using MLXIP antibody 13614-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe, Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 3 publications below
• FC: See 1 publications below
• ChIP: See 5 publications below

Produktinformation

13614-1-AP bindet in WB, IHC, IF/ICC, IP, ChIP, ELISA MLXIP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MLXIP fusion protein Ag4519
• Vollständiger Name: MLX interacting protein
• Berechnetes Molekulargewicht: 919 aa, 100 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC039704
• Gene symbol: MLXIP
• Gene ID (NCBI): 22877
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mlx-interacting protein(MLXIP), after forming heterodimers with MLX, can bind to and then activate transcription from CACGTG E boxes. The N terminus of MLXIP acts as a CRM1-dependent nuclear export signal and contribute to the cytoplasmic localization of the complex, and as a binding site for 13 protein. the C terminus contains a cytoplasmic localization signal and mediates protein-protein interaction of MONDOA and MLX. The complex play a role in transcriptional activation of glycolytic target genes and glucose-responsive gene regulation. This antibody could recognize isoform1(~110kd) and isoform3(~69kd) of MLXIP. A prominent and specific MLXIP of approximately 130 kDa was detected. It demonstrates that MLXIP associated specifically with Mlx in the cytoplasm (PMID: 11073985).

Publikationen

Species	Application	Title
human	WB	Cancer Cell; Deregulated Myc requires MondoA/Mlx for metabolic reprogramming and tumorigenesis.; Authors - Patrick A Carroll
mouse	chIP	Nat Neurosci; PMID: 31636448; Authors - Hei-Man Chow
mouse	WB,FC,ChIP	Gastroenterology; MondoA-Thioredoxin-Interacting Protein Axis Maintains Regulatory T-Cell Identity and Function in Colorectal Cancer Microenvironment.; Authors - Ying Lu
human	ChIP	Proc Natl Acad Sci U S A; Metabolic reprogramming in triple-negative breast cancer through Myc suppression of TXNIP.; Authors - Liangliang Shen
human	WB	Diabetes; MondoA is an Essential Glucose Responsive Transcription Factor in Human Pancreatic Beta Cells.; Authors - Paul Richards
human	WB	PLoS Pathog; Latent KSHV Infected Endothelial Cells Are Glutamine Addicted and Require Glutaminolysis for Survival.; Authors - Erica L Sanchez