MLF1IP Polyklonaler Antikörper

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• KD/KO Validated

MLF1IP Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 13186-1-AP

Synonyme

CENPU, CENP-50, CENP50, CENP U(50), CENP U

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Galerie der Validierungsdaten

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  WB analysis using 13186-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13186-1-AP (MLF1IP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13186-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13186-1-AP (MLF1IP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 13186-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 13186-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human gliomas using 13186-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 13186-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 13186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 13186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 13186-1-AP

  Immunofluorescent analysis of HepG2 cells using 13186-1-AP (MLF1IP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Maushodengewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Gliomgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

13186-1-AP bindet in WB, IHC, IF/ICC, ELISA MLF1IP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MLF1IP fusion protein Ag3858
• Vollständiger Name: MLF1 interacting protein
• Berechnetes Molekulargewicht: 418 aa, 48 kDa
• Beobachtetes Molekulargewicht: 46-50 kDa, 60 kDa
• GenBank-Zugangsnummer: BC031520
• Gene symbol: MLF1IP
• Gene ID (NCBI): 79682
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MLF1IP, also named as CENPU, ICEN24, KLIP1 and PBIP1, belongs to the CENP-U/AME1 family. MLF1IP plays an important role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. Moreover, MLF1IP has been shown to be involved in tumorigenesis in several types of cancer including glioblastoma, prostate cancer and luminal breast cancer (PMID: 27378428). MLF1IP has 3 isoforms with the molecular mass of 21, 38 and 47 kDa.

Publikationen

Species	Application	Title
human	WB,IHC	Cell Signal; Overexpression of MLF1IP promotes colorectal cancer cell proliferation through BRCA1/AKT/p27 signaling pathway.; Authors - Yuting Xu ; KD Validated