MNK1 Polyklonaler Antikörper

MNK1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10136-1-AP

Synonyme

MKNK1, MAPK signal-integrating kinase 1, MAP kinase-interacting serine/threonine-protein kinase 1, MAP kinase signal-integrating kinase 1, EC:2.7.11.1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 10136-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10136-1-AP (MNK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 10136-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10136-1-AP (MNK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10136-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10136-1-AP (MNK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10136-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10136-1-AP (MNK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 10136-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10136-1-AP (MNK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 10136-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MNK1 antibody (10136-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, HL-60-Zellen, Maus-Dünndarmgewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

10136-1-AP bindet in WB, IF/ICC, ELISA MNK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MNK1 fusion protein Ag0184
• Vollständiger Name: MAP kinase interacting serine/threonine kinase 1
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 51 kDa, 47 kDa
• GenBank-Zugangsnummer: BC002755
• Gene symbol: MNK1
• Gene ID (NCBI): 8569
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MNK1 (mitogen-activated protein kinase-interacting kinase 1) was discovered as a consequence of bacterial expression libraries screening for proteins regulated by the classical mitogen-activated protein kinases, the ERKs (extracellular signal-regulated kinases). Phosphorylation of Mnk1 has been shown to activate its kinase activity as well as to enhance its binding to the eukaryotic initiation factor 4G (eIF4G) which functions as a scaffolding protein. Human MNK1 arises from an alternatively spliced transcript giving rise to 2 isoforms, longer MNK1a (50 kDa) and a shorter MNK1b variant (38 kDa) that lacks 89 C-terminal amino acids(PMID: 21406405).

Publikationen

Species	Application	Title
human	WB	Int J Mol Sci; Novel HSP90-PI3K Dual Inhibitor Suppresses Melanoma Cell Proliferation by Interfering with HSP90-EGFR Interaction and Downstream Signaling Pathways.; Authors - Qian Zhao
human	WB	J Enzyme Inhib Med Chem; Design, synthesis and molecular mechanisms of novel dual inhibitors of heat shock protein 90/phosphoinositide 3-kinase alpha (Hsp90/PI3Kα) against cutaneous melanoma.; Authors - Feifei Qin
mouse	WB	Chem Biol Drug Des; Caffeic acid-grafted chitooligosaccharides downregulate MAPK and NF-kB in RAW264.7 cells; Authors - Van-Hoai Bui