MIPEP Polyklonaler Antikörper

MIPEP Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 11002-1-AP

Synonyme

HMIP, MIP, MIPEP

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 11002-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11002-1-AP (MIPEP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11002-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11002-1-AP (MIPEP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 11002-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11002-1-AP (MIPEP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 11002-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 11002-1-AP (MIPEP Antibody) at dilution of 1:50.

• 

  IHC staining of human prostate cancer using 11002-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 11002-1-AP (MIPEP Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

11002-1-AP bindet in WB, IHC, ELISA MIPEP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MIPEP fusion protein Ag1451
• Vollständiger Name: mitochondrial intermediate peptidase
• Berechnetes Molekulargewicht: 81 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC009934
• Gene symbol: MIPEP
• Gene ID (NCBI): 4285
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MIPEP(Mitochondrial intermediate peptidase) is also named as MIP and belongs to the peptidase M3 family. It performs the final step in processing a specific class of nuclear-encoded proteins targeted to the mitochondrial matrix or inner membrane. The enzyme is a monomer of 75 kDa with a transit peptide and about 20% of the fmal MIP preparation consists of equimolar amounts of two peptides of 47 kDa and 28 kDa, which are apparently the products of a single cleavage of the 75 kDa protein. MIP is an independent endopeptidase acting on mitochondrial intermediate proteins as substrates.(PMID:1322290).

Publikationen

Species	Application	Title
human	WB	Biochem Biophys Res Commun; Mitochondrial intermediate peptidase: expression in Escherichia coli and improvement of its enzymatic activity detection with FRET substrates.; Authors - Marcondes Marcelo F MF