MINA Polyklonaler Antikörper

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• KD/KO Validated

MINA Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 12214-1-AP

Synonyme

MDIG, MINA, Mina53, MYC induced nuclear antigen, NO52, Nucleolar protein 52, Ribosomal oxygenase 2, RIOX2, ROX

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 12214-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12214-1-AP (MINA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12214-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12214-1-AP (MINA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 12214-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12214-1-AP (MINA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12214-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12214-1-AP (MINA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 12214-1-AP

  IP result of anti-MINA (IP:12214-1-AP, 3ug; Detection:12214-1-AP 1:500) with HEK-293 cells lysate 2120ug.

• 

  IHC staining of human pancreas cancer using 12214-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12214-1-AP (MINA antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A375-Zellen, A431-Zellen, Mauslebergewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

12214-1-AP bindet in WB, IP, IF, IHC, ELISA MINA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MINA fusion protein Ag2838
• Vollständiger Name: MYC induced nuclear antigen
• Berechnetes Molekulargewicht: 465 aa, 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC014928
• Gene symbol: MINA
• Gene ID (NCBI): 84864
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MINA, also named as MDIG, MINA53 and NO52, belongs to the MINA53/NO66 family. It is involved in cellular proliferation. MINA may play an important role in cell growth and survival. It is involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles. The transcriptional repressor MINA has been shown to repress Il4 gene expression by directly binding to its promoter, thereby, controlling TH2 bias.(PMID:21499227)

Publikationen

Species	Application	Title
mouse	WB,IF	Signal Transduct Target Ther; MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration; Authors - Jinpeng Du ; KD Validated
mouse	WB	EMBO J; NFIL3/E4BP4 controls type 2 T helper cell cytokine expression.; Authors - Kashiwada Masaki M
human	WB	Cancer Res; ZNF143-Mediated H3K9 Trimethylation Upregulates CDC6 by Activating MDIG in Hepatocellular Carcinoma.; Authors - Lili Zhang
human	WB	Oncol Lett; Effects of mdig on proliferation and apoptosis of lung cancer cells.; Authors - Xiaohui Xu ; KD Validated