MICAL2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MICAL2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Rezensionen (1)

Kat-Nr. : 13965-1-AP

Synonyme

MICAL2PV1, MICAL-2, MICAL C-terminal-like protein, KIAA0750, EC:1.14.13.225

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Galerie der Validierungsdaten

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  WB analysis using 13965-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13965-1-AP (MICAL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 13965-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13965-1-AP (MICAL2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 13965-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13965-1-AP (MICAL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13965-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13965-1-AP (MICAL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13965-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13965-1-AP (MICAL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 13965-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using MICAL2 antibody (13965-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 13965-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MICAL2 antibody (13965-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, PC-3-Zellen, U-251-Zellen, U-87 MG-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

13965-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA MICAL2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MICAL2 fusion protein Ag4950
• Vollständiger Name: microtubule associated monoxygenase, calponin and LIM domain containing 2
• Berechnetes Molekulargewicht: 127 kDa
• Beobachtetes Molekulargewicht: 95 kDa, 112 kDa
• GenBank-Zugangsnummer: BC044577
• Gene symbol: MICAL2
• Gene ID (NCBI): 9645
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions. There are three known isoforms, MICAL1, MICAL2, and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL2 has three conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain, and a Lin11, Isl-1, and Mec-3 (LIM) domain. It has been demonstrated that MICAL2 could regulate actin stress fibers and is required for normal actin organization. In addition, MICAL2-PV, a novel splicing variant of MICAL2, has been reported to be involved in cancer progression of prostate cancer. This antibody can recognize both MICAL2 and MICAL2-PV. This antibody recognizes various isoforms of MICAL2 around 90-95 kDa or 109-112 kDa.

Publikationen

Species	Application	Title
human	WB	Theranostics; Renal tubular Bim mediates the tubule-podocyte crosstalk via NFAT2 to induce podocyte cytoskeletal dysfunction.; Authors - Chunmei Xu
human	WB,CoIP,IHC,IF	Oncogene; Phosphorylation of MICAL2 by ARG promotes head and neck cancer tumorigenesis by regulating skeletal rearrangement; Authors - Ze Zhang ; KD Validated
		Cancer Lett; MICAL2 is a novel nucleocytoplasmic shuttling protein promoting cancer invasion and growth of lung adenocarcinoma.; Authors - Wolong Zhou
human	WB	Oxid Med Cell Longev; MICAL2 Contributes to Gastric Cancer Cell Proliferation by Promoting YAP Dephosphorylation and Nuclear Translocation; Authors - Chenxiang Qi ; KD Validated
human	WB	Acta Physiol (Oxf); MICAL2 Promotes Breast Cancer Cell Migration by Maintaining EGFR Stability and EGFR/P38 Signaling Activation.; Authors - Yueyuan Wang ; KD Validated
human	WB	Front Mol Biosci; MICAL2 Facilitates Gastric Cancer Cell Migration via MRTF-A-Mediated CDC42 Activation.; Authors - Yueyuan Wang ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kincaid (Verified Customer) (06-02-2024)

H4 cell lysate run on SDS-PAGE gel. aMICAL2 diluted in 3% BSA, in TBS-tween (0.1%).

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: H4 cells

MICAL2 Antibody Western Blot validation (1:500 dilution) in H4 cells (Cat no:13965-1-AP)