MEN1 Polyklonaler Antikörper

MEN1 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15159-1-AP

Synonyme

MEAI, MEN1, Menin, multiple endocrine neoplasia I, SCG2

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Galerie der Validierungsdaten

• 

  WB analysis of HepG2 using 15159-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15159-1-AP (MEN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15159-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15159-1-AP (MEN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15159-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15159-1-AP (MEN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 15159-1-AP

  IP result of anti-MEN1(IP:15159-1-AP, 4ug; Detection:15159-1-AP 1:500) with HEK-293 cells lysate 1320 ug.

• 

  IF Staining of HeLa using 15159-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15159-1-AP (MEN1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15159-1-AP bindet in WB, IP, IF, ELISA MEN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MEN1 fusion protein Ag7253
• Vollständiger Name: multiple endocrine neoplasia I
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 63-70 kDa
• GenBank-Zugangsnummer: BC002544
• Gene symbol: MEN1
• Gene ID (NCBI): 4221
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MEN1, also named as Menin or MEAI, is a tumor suppressor protein encoded by the MEN1 gene. Its inactivation has been implicated in tumors of many endocrine tissues. Germline inactivating mutations of MEN1 gene are the main cause of multiple endocrine neoplasia type 1 (MEN1), a syndrome characterized by tumors in the pituitary gland, the parathyroid glands, and the enteropancreatic neuroendocrine tissues. Recent study shows astroglia menin deficiency leads to depressive-like behaviors in mice

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