MEIS2 Polyklonaler Antikörper

MEIS2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 11550-1-AP

Synonyme

Homeobox protein Meis2, HsT18361, Meis homeobox 2, Meis1 related protein 1, MEIS2, MRG1

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Galerie der Validierungsdaten

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  WB analysis using 11550-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11550-1-AP (MEIS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11550-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11550-1-AP (MEIS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 11550-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11550-1-AP (MEIS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 11550-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11550-1-AP (MEIS2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 11550-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11550-1-AP (MEIS2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A549-Zellen, HCT 116-Zellen, LNCaP-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

11550-1-AP bindet in WB, IHC, ELISA MEIS2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MEIS2 fusion protein Ag2096
• Vollständiger Name: Meis homeobox 2
• Berechnetes Molekulargewicht: 381 aa, 42 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC001516
• Gene symbol: MEIS2
• Gene ID (NCBI): 4212
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MEIS2, also named as Meis1-related protein 1 or MRG1, is a 477 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the TALE/MEIS homeobox family. MEIS2 is expressed in various tissues and at high level in the lymphoid organs of hematopoietic tissues. MEIS2 localizes in the nucleus and is involved in transcriptional regulation. MEIS2 is a critical downstream effector within an essential homeoprotein network that serving a rate-limiting regulatory role in MLL leukemogenesis. MEIS2 as midbrain specific marker is upregulated concomitantly with the morphological transformation of hindbrain to midbrain.

Publikationen

Species	Application	Title
mouse	IHC	Proc Natl Acad Sci U S A; Congenital diaphragmatic hernia candidate genes derived from embryonic transcriptomes.; Authors - Russell Meaghan K MK
mouse	WB	Aging Cell; Myeloid ectopic viral integration site 2 accelerates the progression of Alzheimer's disease; Authors - Yuting Cui ; KD Validated