MCRS1 Polyklonaler Antikörper

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• KD/KO Validated

MCRS1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, CoIP, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 11362-1-AP

Synonyme

58 kDa microspherule protein, ICP22BP, INO80 complex subunit J, INO80Q, MCRS1, MCRS2, microspherule protein 1, MSP58, P78

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 11362-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 11362-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11362-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11362-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 11362-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 11362-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse uterus using 11362-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 11362-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 11362-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11362-1-AP (MCRS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, humanes Hirngewebe, humanes Lebergewebe, humanes Milzgewebe, Mausmilzgewebe, Maus-Uterusgewebe, Rattenmilzgewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

11362-1-AP bindet in WB, IF, IHC, CoIP, chIP, ELISA MCRS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MCRS1 fusion protein Ag1904
• Vollständiger Name: microspherule protein 1
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC011794
• Gene symbol: MCRS1
• Gene ID (NCBI): 10445
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MCRS1, also named INO80Q, MSP58 or p78, is a nucleolar protein expressed in the very early S phase. It modulates the transcription repressor activity of DAXX by recruiting it to the nucleolus. MCRS1 is also present on polyribosomes of synaptoneurosome as a novel RNA-binding protein to activate the rRNA transcription. The forkhead-associated domain of MCRS1 is involved in association with centrosomal protein Nde1, comprising a component of the centrosome and taking an essential role in viability. Its isoform MCRS2 might be a linker between telomere maintenance and cell-cycle regulation by interacting with LPTS/PinX1, a telomerase-inhibitory protein. MCRS1 is detected by Western blot as a highly modified protein at 78 kDa and minor bands at about 62 and 55 kDa.

Publikationen

Species	Application	Title
human	WB	Cell; MOF Acetyl Transferase Regulates Transcription and Respiration in Mitochondria.; Authors - Aindrila Chatterjee
mouse	WB	Elife; MOF-associated complexes ensure stem cell identity and Xist repression.; Authors - Tomasz Chelmicki
mouse	WB,IF,CoIP	EMBO Rep; Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis; Authors - Jia-Qian Ju ; KO Validated
human	IF	Sci Rep; MCRS1 associates with cytoplasmic dynein and mediates pericentrosomal material recruitment.; Authors - Si-Hyung Lee
human	WB	J Biol Chem; Subunit organization of the human INO80 chromatin remodeling complex: an evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling.; Authors - Chen Lu L
human	WB	Mol Biol Cell; Mps1 regulates spindle morphology through MCRS1 to promote chromosome alignment.; Authors - Hongdan Yang