MCM5 Polyklonaler Antikörper

MCM5 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Rezensionen (1)

Kat-Nr. : 11703-1-AP

Synonyme

P1-CDC46, P1 CDC46, EC:3.6.4.12, CDC46

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Galerie der Validierungsdaten

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  WB analysis using 11703-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11703-1-AP (MCM5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11703-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11703-1-AP (MCM5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 11703-1-AP

  IP result of anti-MCM5 (IP:11703-1-AP, 4ug; Detection:11703-1-AP 1:4000) with Jurkat cells lysate 2400 ug.

• 

  IHC staining of human lymphoma using 11703-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11703-1-AP (MCM5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 11703-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11703-1-AP (MCM5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 11703-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCM5 antibody (11703-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HeLa-Zellen, Jurkat-Zellen, Maus-Thymusgewebe, Ratten-Thymusgewebe
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 14 publications below
• IHC: See 6 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

11703-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA MCM5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MCM5 fusion protein Ag2271
• Vollständiger Name: minichromosome maintenance complex component 5
• Berechnetes Molekulargewicht: 82 kDa
• Beobachtetes Molekulargewicht: 82-88 kDa
• GenBank-Zugangsnummer: BC000142
• Gene symbol: MCM5
• Gene ID (NCBI): 4174
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Minichromosome maintenance, S. Cerevisiae, homolog of 5(MCM5), one component of the complex of MCM2-7, which is the putative replicative essential for once per cell cycle DNA replication initiation and elongation in eukaryotic cells. The MCM complex form a hexameric ring through the interaction surfaces of two neighboring subunits.

Publikationen

Species	Application	Title
human	WB,RIP,IP	Mol Ther; Long noncoding RNA lnc-POP1-1 upregulated by VN1R5 promotes cisplatin resistance in head and neck squamous cell carcinoma through interaction with MCM5.; Authors - Yingying Jiang
human	WB	Elife; MCMBP promotes the assembly of the MCM2-7 hetero-hexamer to ensure robust DNA replication in human cells.; Authors - Yuichiro Saito
human	WB	J Nanobiotechnology; Graphene quantum dots rescue angiogenic retinopathy via blocking STAT3/Periostin/ERK signaling.; Authors - Na Zhao
human	WB	Front Oncol; METTL3 Promotes the Progression of Gastric Cancer via Targeting the MYC Pathway.; Authors - Dong-Dong Yang
human	WB	Oncotarget; Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis.; Authors - Yinan Ma
human	WB	Ecotoxicol Environ Saf; The influence of phenolic environmental estrogen on the transcriptome of uterine leiomyoma cells: A whole transcriptome profiling-based analysis.; Authors - Zemin Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hana (Verified Customer) (07-04-2023)

In this immunoprecipitation experiment, we tested the interaction between MCMBP and MCM5 (detected by 11703-1-AP). One can observe that clear interaction between these two proteins as it was reported previously Sedlackova et al, Nature, 2020.

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: human osteosarcoma U2OS

MCM5 Antibody Western Blot,Immunoprecipitation validation (1:2000 dilution) in human osteosarcoma U2OS (Cat no:11703-1-AP)