MBIP Polyklonaler Antikörper

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• KD/KO Validated

MBIP Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 10685-1-AP

Synonyme

MUK-binding inhibitory protein, MUK binding inhibitory protein, MAPK upstream kinase-binding inhibitory protein, MAP3K12-binding inhibitory protein 1, BM-015

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 10685-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10685-1-AP (MBIP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10685-1-AP

  WB result of MBIP antibody (10685-1-AP; 1:600; room temperature for 1.5 hours) with sh-Control and sh-MBIP transfected HEK-293 cells.

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  WB analysis of HEK-293 using 10685-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10685-1-AP (MBIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10685-1-AP

  IP result of anti-MBIP (IP:10685-1-AP, 4ug; Detection:10685-1-AP 1:300) with HEK-293 cells lysate 2800ug.

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  IHC staining of human testis using 10685-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10685-1-AP (MBIP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 10685-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10685-1-AP (MBIP Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 10685-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10685-1-AP (MBIP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 10685-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10685-1-AP (MBIP Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 10685-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10685-1-AP (MBIP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below

Produktinformation

10685-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA MBIP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MBIP fusion protein Ag1061
• Vollständiger Name: MAP3K12 binding inhibitory protein 1
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC005197
• Gene symbol: MBIP
• Gene ID (NCBI): 51562
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MBIP (MAP3K12 binding inhibitory protein 1), also known as MAPK upstream kinase (MUK)-binding inhibitory protein, is a 344 amino acid nuclear and cytoplasmic protein that assists in the activation of the JNK pathway by inhibiting ZPK activity. Ubiquitously expressed, MBIP is found at high levels in lung and heart and is one component of the ADA2A-containing complex (ATAC) in conjunction with ADA3, GCN5, CDC101, TADA2L, WDR5, YEATS2, DR1 and CRP2BP.

Publikationen

Species	Application	Title
human	WB	Nature; Characterizing the cancer genome in lung adenocarcinoma.; Authors - Weir Barbara A BA ; KD Validated
HUMAN	WB	EMBO J; Distinct roles of GCN5/PCAF-mediated H3K9ac and CBP/p300-mediated H3K18/27ac in nuclear receptor transactivation.; Authors - Qihuang Jin
human	WB	Mol Cell Biol; The double-histone-acetyltransferase complex ATAC is essential for mammalian development.; Authors - Guelman Sebastián S
human	WB	Cell Signal; MBIP promotes ESCC metastasis by activating MAPK pathway; Authors - Yanchun Ma
human	WB	J Biol Chem; Endoplasmic reticulum stress-responsive transcription factor ATF6α directs recruitment of the Mediator of RNA polymerase II transcription and multiple histone acetyltransferase complexes.; Authors - Sela Dotan D
human	WB	J Biol Chem; Human ATAC Is a GCN5/PCAF-containing acetylase complex with a novel NC2-like histone fold module that interacts with the TATA-binding protein.; Authors - Yuan-Liang Wang