MARVELD2 Polyklonaler Antikörper

MARVELD2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 13515-1-AP

Synonyme

DFNB49, MARVD2, MARVEL domain containing 2, MARVELD2, MRVLDC2, Tric, Tricellulin

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Galerie der Validierungsdaten

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  WB analysis using 13515-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13515-1-AP (MARVELD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13515-1-AP

  Hela cell lysates were subjected to SDS PAGE followed by western blot with 13515-1-AP (MARVELD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 13515-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13515-1-AP (MARVELD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 13515-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13515-1-AP (MARVELD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 13515-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13515-1-AP (MARVELD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 13515-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13515-1-AP (MARVELD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Mausnierengewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe, humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

13515-1-AP bindet in WB, IF, IHC, ELISA MARVELD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MARVELD2 fusion protein Ag4446
• Vollständiger Name: MARVEL domain containing 2
• Berechnetes Molekulargewicht: 558 aa, 64 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC033689
• Gene symbol: MARVELD2
• Gene ID (NCBI): 153562
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MARVELD2, also named as Tricellulin or TRIC, is a 558 amino acid protein, which contains one MARVEL domain. MARVELD2 localizes the cell membrane and plays a role in the formation of the epithelial barriers. MARVELD2, is a first integral membrane protein that is concentrated at the vertically oriented tight junction strands of tricellular contacts. MARVELD2 may be a component to maintain the integrity for peripheral nervous system myelin function and morphology.

Publikationen

Species	Application	Title
mouse	WB	J Clin Invest; Tricellulin deficiency affects tight junction architecture and cochlear hair cells.; Authors - Nayak Gowri G
pig	WB,IHC	Microb Pathog; Alterations in the expression level of visfatin in the lungs of piglets infected with PRRSV and its effect on PRRSV replication.; Authors - Zhe-Wei Zhang
	IF	PLoS One; Lectin binding and gel secretion within Lorenzinian electroreceptors of Polyodon; Authors - David F. Russell
	IF	Tissue Cell; Papilla cells may guard the entrance to ampullary organs of Polyodon electroreceptors; Authors - David F Russell
human		Cell Rep; Alpha-tocopherylquinone differentially modulates claudins to enhance intestinal epithelial tight junction barrier via AhR and Nrf2 pathways; Authors - Ashwinkumar Subramenium Ganapathy

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Beth (Verified Customer) (10-05-2017)

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: RWPE1, PC-3, primary prostate epithelial cells

MARVELD2 Antibody Western Blot validation (1:1000 dilution) in RWPE1, PC-3, primary prostate epithelial cells (Cat no:13515-1-AP)